학술논문
A validated restriction enzyme ddPCR cg05575921 (AHRR) assay to accurately assess smoking exposure
Document Type
article
Source
Clinical Epigenetics, Vol 16, Iss 1, Pp 1-7 (2024)
Subject
Language
English
ISSN
1868-7083
Abstract
Abstract Background & Methods In this study, a novel restriction enzyme (RE) digestion-based droplet digital polymerase chain reaction (ddPCR) assay was designed for cg005575921 within the AHRR gene body and compared with matching results obtained by bisulfite conversion (BIS) ddPCR and Illumina DNA methylation array. Results The RE ddPCR cg05575921 assay appeared concordant with BIS ddPCR (r 2 = 0.94, P