학술논문
SARS-CoV-2 genomic surveillance in wastewater as a model for monitoring evolution of endemic viruses
Document Type
article
Author
Mukhlid Yousif; Said Rachida; Setshaba Taukobong; Nkosenhle Ndlovu; Chinwe Iwu-Jaja; Wayne Howard; Shelina Moonsamy; Nompilo Mhlambi; Sipho Gwala; Joshua I. Levy; Kristian G. Andersen; Cathrine Scheepers; Anne von Gottberg; Nicole Wolter; Jinal N. Bhiman; Daniel Gyamfi Amoako; Arshad Ismail; Melinda Suchard; Kerrigan McCarthy
Source
Nature Communications, Vol 14, Iss 1, Pp 1-9 (2023)
Subject
Language
English
ISSN
2041-1723
Abstract
Abstract As global SARS-CoV-2 burden and testing frequency have decreased, wastewater surveillance has emerged as a key tool to support clinical surveillance efforts. The aims of this study were to identify and characterize SARS-CoV-2 variants in wastewater samples collected from urban centers across South Africa. Here we show that wastewater sequencing analyses are temporally concordant with clinical genomic surveillance and reveal the presence of multiple lineages not detected by clinical surveillance. We show that wastewater genomics can support SARS-CoV-2 epidemiological investigations by reliably recovering the prevalence of local circulating variants, even when clinical samples are not available. Further, we find that analysis of mutations observed in wastewater can provide a signal of upcoming lineage transitions. Our study demonstrates the utility of wastewater genomics to monitor evolution and spread of endemic viruses.