학술논문
The uterine and vascular actions of estetrol delineate a distinctive profile of estrogen receptor α modulation, uncoupling nuclear and membrane activation
Document Type
article
Author
Anne Abot; Coralie Fontaine; Mélissa Buscato; Romain Solinhac; Gilles Flouriot; Aurélie Fabre; Anne Drougard; Shyamala Rajan; Muriel Laine; Alain Milon; Isabelle Muller; Daniel Henrion; Marine Adlanmerini; Marie‐Cécile Valéra; Anne Gompel; Céline Gerard; Christel Péqueux; Mélanie Mestdagt; Isabelle Raymond‐Letron; Claude Knauf; François Ferriere; Philippe Valet; Pierre Gourdy; Benita S Katzenellenbogen; John A Katzenellenbogen; Françoise Lenfant; Geoffrey L Greene; Jean‐Michel Foidart; Jean‐François Arnal
Source
EMBO Molecular Medicine, Vol 6, Iss 10, Pp 1328-1346 (2014)
Subject
Language
English
ISSN
1757-4684
1757-4676
1757-4676
Abstract
Abstract Estetrol (E4) is a natural estrogen with a long half‐life produced only by the human fetal liver during pregnancy. The crystal structures of the estrogen receptor α (ERα) ligand‐binding domain bound to 17β‐estradiol (E2) and E4 are very similar, as well as their capacity to activate the two activation functions AF‐1 and AF‐2 and to recruit the coactivator SRC3. In vivo administration of high doses of E4 stimulated uterine gene expression, epithelial proliferation, and prevented atheroma, three recognized nuclear ERα actions. However, E4 failed to promote endothelial NO synthase activation and acceleration of endothelial healing, two processes clearly dependent on membrane‐initiated steroid signaling (MISS). Furthermore, E4 antagonized E2 MISS‐dependent effects in endothelium but also in MCF‐7 breast cancer cell line. This profile of ERα activation by E4, uncoupling nuclear and membrane activation, characterizes E4 as a selective ER modulator which could have medical applications that should now be considered further.