학술논문
Comprehensive landscape of neutralizing antibody and cell-mediated response elicited by the 1/5 fractional dose of 17DD-YF primary vaccination in adults
Document Type
article
Author
Laise Rodrigues Reis; Ismael Artur Costa-Rocha; Thais Abdala-Torres; Ana Carolina Campi-Azevedo; Vanessa Peruhype-Magalhães; Márcio Sobreira Silva Araújo; Elaine Spezialli; Lis Ribeiro do Valle Antonelli; Rosiane Aparecida da Silva-Pereira; Gregório Guilherme Almeida; Eder Gatti Fernandes; Francieli Fontana Sutile Tardetti Fantinato; Carla Magda Allan Santos Domingues; Maria Cristina Ferreira Lemos; Alexandre Chieppe; Jandira Aparecida Campos Lemos; Jordana Grazziela Coelho-dos-Reis; Sheila Maria Barbosa de Lima; Adriana de Souza Azevedo; Waleska Dias Schwarcz; Luiz Antônio Bastos Camacho; Maria de Lourdes de Sousa Maia; Tatiana Guimarães de Noronha; Caroline Duault; Yael Rosenberg-Hasson; Andréa Teixeira-Carvalho; Holden Terry Maecker; Olindo Assis Martins-Filho; Collaborative Group for Studies of Yellow Fever Vaccine
Source
Scientific Reports, Vol 14, Iss 1, Pp 1-18 (2024)
Subject
Language
English
ISSN
2045-2322
Abstract
Abstract The present study aimed at evaluating the YF-specific neutralizing antibody profile besides a multiparametric analysis of phenotypic/functional features of cell-mediated response elicited by the 1/5 fractional dose of 17DD-YF vaccine, administered as a single subcutaneous injection. The immunological parameters of each volunteer was monitored at two time points, referred as: before (Day 0) [Non-Vaccinated, NV(D0)] and after vaccination (Day 30–45) [Primary Vaccinees, PV(D30–45)]. Data demonstrated high levels of neutralizing antibodies for PV(D30–45) leading to a seropositivity rate of 93%. A broad increase of systemic soluble mediators with a mixed profile was also observed for PV(D30–45), with IFN-γ and TNF-α presenting the highest baseline fold changes. Integrative network mapping of soluble mediators showed increased correlation numbers in PV(D30–45) as compared to NV(D0) (532vs398). Moreover, PV(D30–45) exhibited increased levels of Terminal Effector (CD45RA+CCR7−) CD4+ and CD8+ T-cells and Non-Classical memory B-cells (IgD+CD27+). Dimensionality reduction of Mass Cytometry data further support these findings. A polyfunctional cytokine profile (TNF-α/IFN-γ/IL-10/IL-17/IL-2) of T and B-cells was observed upon in vitro antigen recall. Mapping and kinetics timeline of soluble mediator signatures for PV(D30–45) further confirmed the polyfunctional profile upon long-term in vitro culture, mediated by increased levels of IFN-γ and TNF-α along with decreased production of IL-10. These findings suggest novel insights of correlates of protection elicited by the 1/5 fractional dose of 17DD-YF vaccine.