학술논문
SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e
Document Type
article
Source
Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)
Subject
Language
English
ISSN
2041-1723
Abstract
Increased-fidelity SpCas9 variants have been developed, but often show proportionally reduced activity. Here the authors characterise the on-target activity and off-target propensity of SuperFi-Cas9 in mammalian cells and also see strongly reduced activity but with high fidelity features.