학술논문
Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions
Document Type
article
Author
Daniel Butler; Christopher Mozsary; Cem Meydan; Jonathan Foox; Joel Rosiene; Alon Shaiber; David Danko; Ebrahim Afshinnekoo; Matthew MacKay; Fritz J. Sedlazeck; Nikolay A. Ivanov; Maria Sierra; Diana Pohle; Michael Zietz; Undina Gisladottir; Vijendra Ramlall; Evan T. Sholle; Edward J. Schenck; Craig D. Westover; Ciaran Hassan; Krista Ryon; Benjamin Young; Chandrima Bhattacharya; Dianna L. Ng; Andrea C. Granados; Yale A. Santos; Venice Servellita; Scot Federman; Phyllis Ruggiero; Arkarachai Fungtammasan; Chen-Shan Chin; Nathaniel M. Pearson; Bradley W. Langhorst; Nathan A. Tanner; Youngmi Kim; Jason W. Reeves; Tyler D. Hether; Sarah E. Warren; Michael Bailey; Justyna Gawrys; Dmitry Meleshko; Dong Xu; Mara Couto-Rodriguez; Dorottya Nagy-Szakal; Joseph Barrows; Heather Wells; Niamh B. O’Hara; Jeffrey A. Rosenfeld; Ying Chen; Peter A. D. Steel; Amos J. Shemesh; Jenny Xiang; Jean Thierry-Mieg; Danielle Thierry-Mieg; Angelika Iftner; Daniela Bezdan; Elizabeth Sanchez; Thomas R. Campion; John Sipley; Lin Cong; Arryn Craney; Priya Velu; Ari M. Melnick; Sagi Shapira; Iman Hajirasouliha; Alain Borczuk; Thomas Iftner; Mirella Salvatore; Massimo Loda; Lars F. Westblade; Melissa Cushing; Shixiu Wu; Shawn Levy; Charles Chiu; Robert E. Schwartz; Nicholas Tatonetti; Hanna Rennert; Marcin Imielinski; Christopher E. Mason
Source
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
Subject
Language
English
ISSN
2041-1723
Abstract
Here, using clinical samples and autopsy tissues, the authors combine fast-colorimetric test (LAMP) for SARS-CoV-2 infection and large-scale shotgun metatranscriptomics for host, viral, and microbial profiling and provide a map of the viral genetic features of the New York City outbreak and associate specific host responses and gene expression perturbations with SARS-CoV-2 infection.