학술논문
Integrated phenotyping of the anti-cancer immune response in HIV-associated hepatocellular carcinoma
Document Type
article
Author
David J. Pinato; Takahiro Kaneko; Antonio D’Alessio; Alejandro Forner; Petros Fessas; Beatriz Minguez; Edoardo G. Giannini; Federica Grillo; Alba Díaz; Francesco A. Mauri; Claudia A.M. Fulgenzi; Alessia Dalla Pria; Robert D. Goldin; Giulia Pieri; Pierluigi Toniutto; Claudio Avellini; Maria Corina Plaz Torres; Ayse U. Akarca; Teresa Marafioti; Sherrie Bhoori; Jose María Miró; Mark Bower; Norbert Bräu; Vincenzo Mazzaferro
Source
JHEP Reports, Vol 5, Iss 7, Pp 100741- (2023)
Subject
Language
English
ISSN
2589-5559
Abstract
Background & Aims: HIV-seropositivity shortens survival in patients with hepatocellular carcinoma (HCC). Although risk factors for HCC including HCV infection can influence T cell phenotype, it is unknown whether HIV can influence functional characteristics of the T cell infiltrate. Methods: From the Liver Cancer in HIV biorepository, we derived 129 samples of transplanted (76%) or resected (20%) HCC in eight European and North American centres. We profiled intra- and peritumoural tissue to evaluate regulatory CD4+/FOXP3+ and immune-exhausted CD8+/PD1+ T cells in HIV+ (n = 66) and HIV- (n = 63) samples. We performed targeted transcriptomics and T-cell receptor sequencing in a restricted subset of samples evaluated in relationship with HIV status. We correlated immunopathologic features with patients’ characteristics including markers of HIV infection. Results: Of the 66 HIV+ patients, 83% were HCV coinfected with an undetectable HIV viral load (51%) and a median blood CD4+ cell count of 430 cells/mm3 (range 15–908). Patients who were HIV+ were compared with HIV- controls with similar staging characteristics including Barcelona Clinic Liver Cancer (BCLC) stage A–B (86% vs. 83%, p = 0.16),