학술논문
Serum iron levels in tuberculosis patients and household contacts and its association with natural resistance‐associated macrophage protein 1 polymorphism and expression
Document Type
article
Author
Source
The Clinical Respiratory Journal, Vol 17, Iss 9, Pp 893-904 (2023)
Subject
Language
English
ISSN
1752-699X
1752-6981
1752-6981
Abstract
Abstract Background Iron deficiency can impair immune function, increasing tuberculosis (TB) susceptibility and severity. The research aimed to investigate iron deficiency anemia in TB patients and household contacts and its association with natural resistance‐associated macrophage protein 1 (NRAMP1) polymorphism and expression. Methods The levels of iron, ferritin, and transferrin were measured in the serum by ELISA (Enzyme‐Linked Immunosorbent Assay). NRAMP1 polymorphisms were determined by polymerase chain reaction (PCR) and sequencing. NRAMP1 gene expression was measured by real‐time PCR. Interferon‐gamma release assay (IGRA) checked on household contacts to screen household contacts with positive IGRA as the control. Results This study involved 35 TB cases and 35 TB contacts. The results showed that the serum Fe levels were found to be lower in the TB case group (median 149.6 μmol/L) than in the positive IGRA household contacts group (median 628.53 μmol/L) with a p‐value