학술논문
聚甲基丙烯酸甲酯骨水泥对肺癌细胞毒性作用的体外实验 / In vitro toxic effect of polymethylmethacrylate bone cement on lung cancer cells
Document Type
Academic Journal
Author
潘元星; 米川; 施学东; 王冰; 崔云鹏; 林云飞; Pan Yuan-xing; Mi Chuan; Shi Xue-dong; Wang Bing; Cui Yun-peng; Lin Yun-fei
Source
中国组织工程研究. 21(2):187-191
Subject
Language
Chinese
ISSN
2095-4344
Abstract
背景：经皮椎体成形是治疗脊柱转移瘤的一种微创手术方法，但术中注射的聚甲基丙烯酸甲酯(polymethylmethacrylate，PMMA)骨水泥对肿瘤的治疗机制目前尚不明确。目的：分析PMMA骨水泥及其单体对肿瘤细胞的细胞毒作用。方法：将固化期、拉丝期PMMA骨水泥的浸提液及不同质量浓度单体的稀释液与人肺腺癌细胞spc-A1接触培养1，3 d后，采用细胞形态直接观察法和CCK-8比色法测定细胞吸光度(A)值，计算细胞相对增殖率(RGR)，评价PMMA骨水泥及其单体对肿瘤细胞的毒性。结果与结论：①1 d 1 g/L组、10 g/L组的A值明显小于阴性对照组(P<0.01)；3 d 1 g/L组、10 g/L组及拉丝期浸提液A值明显小于阴性对照组(P<0.05)；②结果显示培养1 d 1 g/L组、10 g/L组的A值明显小于阴性对照组(P<0.01)，细胞相对增殖率为26%-29%，毒性评价为4级；3 d 1 g/L组、10 g/L组的A值明显小于阴性对照组，细胞相对增殖率12%-16%，毒性评价为4至5级；3 d 100 mg/L组、拉丝期PMMA浸提液组的A值明显小于阴性对照组，毒性评价为2级。③结果表明，拉丝期PMMA骨水泥浸提液及PMMA单体质量浓度为100 mg/L-10 g/L时对肿瘤细胞有明显的毒性，说明经皮椎体成形过程中拉丝期PMMA骨水泥注入椎体后其释放的单体可能对肿瘤细胞存在细胞毒作用。
BACKGROUND:Percutaneous vertebroplasty is a minimal y invasive treatment for spinal metastasis tumor, but the mechanism of the injected polymethylmethacrylate (PMMA) bone cement in the treatment of tumor is not ful y understood. OBJECTIVE:To explore the cytotoxicity of PMMA bone cement and its monomers on tumor cel s. METHODS:PMMA extracts in the wire drawing and curing periods and different mass concentrations of monomer dilutions were co-cultured with human lung cancer cel s spc-A1. The cel morphology was observed using inverted microscope, the absorbance (A) values were detected by cel counting kit-8 assay, the relative growth rate was calculated, and the toxicity of PMAA bone cement and its monomers was evaluated at 1 and 3 days of culture, respectively. RESULTS AND CONCLUSION:At 1 day of culture, the absorbance values in the 1 and 10 g/L groups were significantly lower than that in the negative control group (P<0.01). The absorbance values in the 1 g/L, 10 g/L, 100 mg/L and wire drawing extract groups were significantly lower than those in the negative control group at 3 days of culture (P<0.05). The relative growth rate in the 1 and 10 g/L groups was 26%-29%, and the level of toxicity was grade 4 after 1-day culture;at 3 days, the relative growth rate was decreased to 12%-16%, and the level of toxicity was grades 4-5. After 3-day culture, the level of toxicity reached to grade 2 in the 100 mg/L and wire drawing extract groups. These results indicate that PMMA bone cement in wiredrawing period and its monomers exert toxic effects on tumor cels.
BACKGROUND:Percutaneous vertebroplasty is a minimal y invasive treatment for spinal metastasis tumor, but the mechanism of the injected polymethylmethacrylate (PMMA) bone cement in the treatment of tumor is not ful y understood. OBJECTIVE:To explore the cytotoxicity of PMMA bone cement and its monomers on tumor cel s. METHODS:PMMA extracts in the wire drawing and curing periods and different mass concentrations of monomer dilutions were co-cultured with human lung cancer cel s spc-A1. The cel morphology was observed using inverted microscope, the absorbance (A) values were detected by cel counting kit-8 assay, the relative growth rate was calculated, and the toxicity of PMAA bone cement and its monomers was evaluated at 1 and 3 days of culture, respectively. RESULTS AND CONCLUSION:At 1 day of culture, the absorbance values in the 1 and 10 g/L groups were significantly lower than that in the negative control group (P<0.01). The absorbance values in the 1 g/L, 10 g/L, 100 mg/L and wire drawing extract groups were significantly lower than those in the negative control group at 3 days of culture (P<0.05). The relative growth rate in the 1 and 10 g/L groups was 26%-29%, and the level of toxicity was grade 4 after 1-day culture;at 3 days, the relative growth rate was decreased to 12%-16%, and the level of toxicity was grades 4-5. After 3-day culture, the level of toxicity reached to grade 2 in the 100 mg/L and wire drawing extract groups. These results indicate that PMMA bone cement in wiredrawing period and its monomers exert toxic effects on tumor cels.