학술논문
富血小板血浆对大鼠阴茎海绵体神经再生的影响 / Effect of platelet rich plasma on the regeneration of cavernous nerve:experiment with rats
Document Type
Academic Journal
Author
丁协刚; 李世文; 郑新民; 胡礼泉; 胡万里; 罗仪; DING Xie-gang; LI Shi-wen; ZHENG Xin-min; HU Li-quan; HU Wan-li; LUO Yi
Source
中华医学杂志 / NATIONAL MEDICAL JOURNAL OF CHINA. 88(36):2578-2580
Subject
Language
Chinese
ISSN
0376-2491
Abstract
目的 探讨富血小板血浆(PRP)是否促进大鼠海绵体神经再生.方法 24只SD雄性大鼠分成假手术组、双侧海绵体神经切断后立即显微缝合组(单纯缝合组)及缝合后立即局部应用组(PfuP应用组),术后3个月通过电刺激观察海绵体内压的变化来反映其勃起功能,随后取海绵体神经干进行甲苯胺蓝染色,观察轴突的变化来反映神经再生情况.结果 术后3个月,海绵体神经单纯缝合组最大海绵体内压为(46±8)cmH2O,明显低于假手术组(109±13)cmH2O(P＜0.01);而PRP应用组为(94±13)em H2O,明显高于单纯缝合组(P＜0.01).甲苯胺蓝染色PRP应用组轴突个数为(121±16),明显高于单纯缝合组(70±14)(P＜0.01),但仍低于假手术组(181±21)(P＜0.01).结论 局部应用PRP能明显促进受损阴茎海绵体神经的神经再生和勃起功能的恢复.
Objective To investigate the effect of platelet rich plasma(PRP)on the regeneration of injured cavernous nerve(CN).Methods Blood was collected from the hearts of 6 SD rata to prepare PRP.24 male adult rats were randomly divided into 3 equal groups:pure suture group undergoing bilateral CN transaction and pure suture immediately,PRP group undergoing bilateral CN transaction+suture+PRP 200 μl to the site of suture,and sham operation group.3 months later intracavernous pressure(ICP)was measured by CN electrostimulation and then samples of CN were obrained to undergo pathological examination.Results 3 months later after surgery,the ICP of the pure suture group was(46±8)cm H2O,significantly lower than that of the sham group[(109±13)cm H2O,P＜0.01],and that of the PRP group was(94±13)cm H2O,significantly higher than that of the pure sutured group(P＜0.01),however,still significantly lower than that of the sham operation group(P＜0.05).The number of axons of CN in the PRP group was(121±16),significantly higher than that of the pure sutured group(70±14,P＜0.01);however,still significantly lower than that of the sham operation group(181±21,P＜0.01).Conclusion PRP can promote the regeneration of injured CN and the recovery of erectile function.
Objective To investigate the effect of platelet rich plasma(PRP)on the regeneration of injured cavernous nerve(CN).Methods Blood was collected from the hearts of 6 SD rata to prepare PRP.24 male adult rats were randomly divided into 3 equal groups:pure suture group undergoing bilateral CN transaction and pure suture immediately,PRP group undergoing bilateral CN transaction+suture+PRP 200 μl to the site of suture,and sham operation group.3 months later intracavernous pressure(ICP)was measured by CN electrostimulation and then samples of CN were obrained to undergo pathological examination.Results 3 months later after surgery,the ICP of the pure suture group was(46±8)cm H2O,significantly lower than that of the sham group[(109±13)cm H2O,P＜0.01],and that of the PRP group was(94±13)cm H2O,significantly higher than that of the pure sutured group(P＜0.01),however,still significantly lower than that of the sham operation group(P＜0.05).The number of axons of CN in the PRP group was(121±16),significantly higher than that of the pure sutured group(70±14,P＜0.01);however,still significantly lower than that of the sham operation group(181±21,P＜0.01).Conclusion PRP can promote the regeneration of injured CN and the recovery of erectile function.