부산대학교 도서관

Objective:To establish hyperlipemia models in rabbits fed with high fat diet, observe the changes of serum lipid level, and to explore the effect of hyperlipidemia on the proliferation and apoptosis of myocardial cells in rabbits.Methods:New Zealand white male special rabbits were randomly divided into 2 groups:normal diet group and high-fat-fed group, 10 cases in each group. The rabbits in normal group were fed with ordinary feed while the rabbits in the latter group were fed with high fat diet. The levels of serum TC, TG, HDL-C and LDL-C were detected at 0, 4th, 7th, 10th, 13th weeks for 2 groups. At the end of the 13th week, the pathological changes of myocardial tissues were detected by HE staining, and the proliferation and apoptosis of cardiomyocyte were tested by FCM.Results:After given high fat diet, the level of serum TC, TG, HDL-C and LDL-C in high-fat-fed diet group were signiifcantly increased than in the normal diet group from the 4th week to the 13th week (P<0.01). The construction of cardiomyocyte in the atherogenic diet group had obviously morphological changes. The distribution of G0/G1 phase in high-fat-fed increased significantly than that in the normal diet group (P<0.01), but S phase and proliferation index (PI) in decreased signiifcantly than that in the normal diet group (P<0.01). The apoptosis rate in both groups had no statistical difference (P>0.05).Conclusion:Hyperlipidemia can induce the pathological changes of cardiomyocyte in rabbits, inhibit the proliferation of cardiomyocyte, but has no effect on the apoptosis of cardiomycyte in rabbits.