학술논문
漆酶-原儿茶醛交联鱼皮胶原蛋白水凝胶的研究 / Study on the Collagen Hydrogel of Fish Skin via Laccase-Proto-catechuic Aldehyde Cross-linking
Document Type
Academic Journal
Author
Source
皮革科学与工程 / Leather Science and Engineering. 34(2):1-46
Subject
Language
Chinese
ISSN
1004-7964
Abstract
与哺乳动物蛋白相比,鱼皮胶原蛋白未受到克雅氏病和牛海绵状脑病流行的限制,具有较大的应用潜力,然而较差的力学性能和耐降解性质限制了鱼皮胶原的应用.论文探究了漆酶(LAC)和原儿茶醛(PAL)交联制备胶原蛋白水凝胶的交联机理,凝胶微观结构,力学性能和耐降解性质.结果表明,漆酶能够交联胶原成胶.同时,原儿茶醛的引入增强了漆酶交联胶原的作用,提升了胶原蛋白水凝胶的结构稳定性和耐降解性质.LAC-PAL交联胶原是一种构建胶原蛋白水凝胶的有效策略.
Fish skin collagen has greater application potential than mammalian proteins because it is not limited by the prevalence of Creutzfeldt-Jakob disease and bovine spongiform encephalopathy.However,it is restricted by its poor mechanical properties and poor resistance to degradation.This work investigated the cross-linking mechanism,gel microstructure,mechanical properties and degradation resistance of collagen hydrogels prepared by the cross-linking of laccase(LAC)with protocatechuic aldehyde(PAL).Results showed that LAC could cross-link collagen to form a hydrogel.Meanwhile,the introduction of PAL enhanced the role of LAC in cross-linking collagen and improved the structural stability and degradation resistance of the resulting collagen hydrogels.The collagen cross-linked via LAC-PAL is an effective strategy for constructing collagen hydrogels.
Fish skin collagen has greater application potential than mammalian proteins because it is not limited by the prevalence of Creutzfeldt-Jakob disease and bovine spongiform encephalopathy.However,it is restricted by its poor mechanical properties and poor resistance to degradation.This work investigated the cross-linking mechanism,gel microstructure,mechanical properties and degradation resistance of collagen hydrogels prepared by the cross-linking of laccase(LAC)with protocatechuic aldehyde(PAL).Results showed that LAC could cross-link collagen to form a hydrogel.Meanwhile,the introduction of PAL enhanced the role of LAC in cross-linking collagen and improved the structural stability and degradation resistance of the resulting collagen hydrogels.The collagen cross-linked via LAC-PAL is an effective strategy for constructing collagen hydrogels.