학술논문
老年性白内障的氧化損傷與酶組織化學觀察 / Oxidative damage and enzyme histochemistry changes in senile cataract
Document Type
Academic Journal
Author
Source
美中国际眼科杂志 / U.S. CHINESE JOURNAL OF OPHTHALMOLOGY. 1(1):1-3
Subject
Language
Chinese
ISSN
1672-5123
Abstract
目的研究老年性白内障晶狀體上皮酶活性變化和氧化損傷對培養的牛晶狀體上皮細胞酶活性的影響.方法1取老年性白内障晶狀體和正常透明晶狀體進行酶組織化學染色,觀察SDH、LDH、G6PD、ATPase活性的變化.2觀察培養牛晶狀體上皮細胞氧化損傷后及維生素C治療后SDH、LDH、G6PD活性的改變. 結果1老年性白内障SDH、LDH、G6PD、ATPase活性降低.2培養的牛晶狀體上皮細胞經過氧化損傷后SDH、LDH、G6PD活性顯著降低,維生素C可使酶活性顯著提高.結論氧化損傷使能量産生减少,可能是白内障發生的原因之一,維生素C對氧化損傷具有部分保護作用.
To study the possible etiopathology of senile cataract,the changes in the activity of lens epithelial enzyme in senile cataract and the effects of oxidative damage on the enzymatic activity of the cultured bovine lens epithelial cells. Methods 1. The activities of succinate dehydrogenase ( SDH)、 Lactate dehydrogenase ( LDH)、 Glucose - 6 - phosphate dehydrogenase (G60D) and Adenosine triphosphatase (ATPase) collected from senile cataract and normal lens epithelial cells were observed by enzyme histochemistry methods. 2. An experimental oxidative damage model of cultured bovine lens epithelial cells in vitro was established. Then the activities of SDH、 LDH and G6PD were observed after oxidative damage and then,after VitC treatment. Results 1. The activities of SDH、 LDH、 G6PD and ATPase of senile cataract lens epithelial cells were apparently lower than those of normal transparent lens.2. After oxidative damage, the activities of SDH、 LDH and G6PD of cultured bovine lens epithelial cells dramatically decreased. Vitamin C partly raised the enzyme activities, rather than bring them to normal.Conclusion Activities of enzyme related with energetic metabolism are decreased following oxidative damage, which may be a possible cause of senile cataract.
To study the possible etiopathology of senile cataract,the changes in the activity of lens epithelial enzyme in senile cataract and the effects of oxidative damage on the enzymatic activity of the cultured bovine lens epithelial cells. Methods 1. The activities of succinate dehydrogenase ( SDH)、 Lactate dehydrogenase ( LDH)、 Glucose - 6 - phosphate dehydrogenase (G60D) and Adenosine triphosphatase (ATPase) collected from senile cataract and normal lens epithelial cells were observed by enzyme histochemistry methods. 2. An experimental oxidative damage model of cultured bovine lens epithelial cells in vitro was established. Then the activities of SDH、 LDH and G6PD were observed after oxidative damage and then,after VitC treatment. Results 1. The activities of SDH、 LDH、 G6PD and ATPase of senile cataract lens epithelial cells were apparently lower than those of normal transparent lens.2. After oxidative damage, the activities of SDH、 LDH and G6PD of cultured bovine lens epithelial cells dramatically decreased. Vitamin C partly raised the enzyme activities, rather than bring them to normal.Conclusion Activities of enzyme related with energetic metabolism are decreased following oxidative damage, which may be a possible cause of senile cataract.