학술논문
变叶榕提取物不同组分总黄酮含量及抗氧化抗炎活性研究 / Total Flavonoids Contents,Antioxidant and Anti-inflammatory Activities of Different Fractions of Ficus variolosa Extraction
Document Type
Academic Journal
Author
吴文明; 习丹; 邹婷婷; 熊贻友; 应萍; 胡建新; WU Wen-ming; XI Dan; ZOU Ting-ting; XIONG Yi-you; YING Ping; HU Jian-xin
Source
广州化工 / Guangzhou Chemical Industry. 51(19):38-54
Subject
Language
Chinese
ISSN
1001-9677
Abstract
采用NaNO2-Al(NO3)3-NaOH法测定各组分总黄酮含量;DPPH、ABTS自由基清除及Fe3+还原能力试验测定各组分抗氧化活性;Griees试剂法评价各组分抗炎活性.研究发现,变叶榕根提取物总黄酮含量为(161.26±2.74)mg/g,乙酸乙酯组分总黄酮含量最高,为(285.75±5.99)mg/g.乙酸乙酯组分对DPPH、ABTS自由基清除和Fe3+还原能力的IC50 值分别为15.53、6.69和56.52 μg/mL,对LPS诱导RAW264.7 细胞释放NO的抑制活性的IC50 值为63.07 μg/mL.结果表明,变叶榕根乙酸乙酯组分具有较明显的抗氧化抗炎活性,其化学成分及作用机制值得进一步研究.
The contents of total flavonoids in variousfractions were determined by NaNO2-Al(NO3)3-NaOH assay.Antioxidant activity of the different fractions were evaluated by DPPH,ABTS free radical scavenging and reducing power measurement,and anti-inflammatory activity were evaluated by measuring NO that released from mice's abdominal macrophage RAW264.7 induced by lipopolysaccharide.The results revealed that the content of total flavonoids of ethanol extraction of Ficus variolosa roots was(161.26±2.74)μg/mL.The content of total flavonoids of ethyl acetate fraction was(285.75±5.99)μg/mL,which was significantly higher than that of any other fractions.EtOAc fraction had the most potent anti-oxidative and anti-inflammatory effect.IC50 values for EtOAc fraction against DPPH,ABTS free radical,and reducing power were 15.53,6.69 and 56.52 μg/mL,respectively.The IC50 values of the inhibitory activities of LPS-induced NO release in RAW264.7 cells was 63.07 μg/mL.EtOAc fraction had obvious antioxidant and anti-inflammatory activities,and its chemical composition and mechanism of action deserved further study.
The contents of total flavonoids in variousfractions were determined by NaNO2-Al(NO3)3-NaOH assay.Antioxidant activity of the different fractions were evaluated by DPPH,ABTS free radical scavenging and reducing power measurement,and anti-inflammatory activity were evaluated by measuring NO that released from mice's abdominal macrophage RAW264.7 induced by lipopolysaccharide.The results revealed that the content of total flavonoids of ethanol extraction of Ficus variolosa roots was(161.26±2.74)μg/mL.The content of total flavonoids of ethyl acetate fraction was(285.75±5.99)μg/mL,which was significantly higher than that of any other fractions.EtOAc fraction had the most potent anti-oxidative and anti-inflammatory effect.IC50 values for EtOAc fraction against DPPH,ABTS free radical,and reducing power were 15.53,6.69 and 56.52 μg/mL,respectively.The IC50 values of the inhibitory activities of LPS-induced NO release in RAW264.7 cells was 63.07 μg/mL.EtOAc fraction had obvious antioxidant and anti-inflammatory activities,and its chemical composition and mechanism of action deserved further study.