학술논문
ε--聚赖氨酸生产菌育种研究进展 / Advances in Strain Improvement for the Production of ε-Poly-L-lysine
Document Type
Academic Journal
Author
孙子龙; 扶教龙; 王月; 周运峰; 李博彦; 钱玮; 白净; 鞠鑫; 李良智; SUN Zilong; FU Jiaolong; WANG Yue; ZHOU Yunfeng; LI Boyan; QIAN Wei; BAI Jing; JU Xin; LI Liangzhi
Source
食品科学 / Food Science. 44(23):231-239
Subject
Language
Chinese
ISSN
1002-6630
Abstract
ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)是一种含25～35个L-赖氨酸残基的新型生物聚合物,由ε-NH2和α-COOH脱水缩合形成.其具有热稳定性、可食性、水溶性、可降解性、抑菌谱广及无毒等优异性能,作为一种安全的食品防腐剂,在日本、韩国、美国、中国和一些其他国家得以应用.ε-PL通常由白色链霉菌(Streptomyces albulus)发酵生产,对生产菌进行育种改造是提高ε-PL生产和降低成本的关键方法之一.目前,研究者们己通过理化诱变、核糖体工程、基因组重排、基因工程等方法对ε-PL生产菌进行了大量育种研究,且得到了一些高产菌株.本文首先简要介绍ε-PL生物合成机理,并从上述几个方面着重介绍c-PL生产菌育种研究进展,然后概述ε-PL发酵生产工艺,最后对相关研究进行展望,以期为ε-PL微生物育种和绿色生物制造提供借鉴与参考.
ε-Poly-L-lysine(ε-PL)is a novel biopolymer consisting of 25-35 L-lysine residues,which is formed by the dehydration condensation of ε-NH2 and a-COOH.ε-PL possesses many excellent characteristics,such as antimicrobial activity,edibility,water solubility,biodegradability,thermostability and nontoxicity.As a natural and safe food preservative,ε-PL possesses many excellent advantages such as thermal stability,edibility,water.solubility,degradability,and broad-spectrum antibacterial activity and has been successfully utilized in Japan,South Korea,the United States,China and other countries.ε-PL is usually produced by fermentation with Streptomyces albulus,and improving ε-PL-producing stains is crucial for enhancing ε-PL production and reducing costs.At present,researchers have obtained microbial strains capable of producing high levels of ε-PL by using physicochemical mutagenesis,ribosome engineering,genome shuffling,genetic engineering and other methods.This review introduces the mechanism of ε-PL biosynthesis and recent progress in strain improvement for the production of ε-PL,and gives an overview of the fermentation process of ε-PL.Finally,this review concludes with an outlook on future research directions.We hope that this review can help promote strain improvement for green biological manufacturing of ε-poly-L-lysine.
ε-Poly-L-lysine(ε-PL)is a novel biopolymer consisting of 25-35 L-lysine residues,which is formed by the dehydration condensation of ε-NH2 and a-COOH.ε-PL possesses many excellent characteristics,such as antimicrobial activity,edibility,water solubility,biodegradability,thermostability and nontoxicity.As a natural and safe food preservative,ε-PL possesses many excellent advantages such as thermal stability,edibility,water.solubility,degradability,and broad-spectrum antibacterial activity and has been successfully utilized in Japan,South Korea,the United States,China and other countries.ε-PL is usually produced by fermentation with Streptomyces albulus,and improving ε-PL-producing stains is crucial for enhancing ε-PL production and reducing costs.At present,researchers have obtained microbial strains capable of producing high levels of ε-PL by using physicochemical mutagenesis,ribosome engineering,genome shuffling,genetic engineering and other methods.This review introduces the mechanism of ε-PL biosynthesis and recent progress in strain improvement for the production of ε-PL,and gives an overview of the fermentation process of ε-PL.Finally,this review concludes with an outlook on future research directions.We hope that this review can help promote strain improvement for green biological manufacturing of ε-poly-L-lysine.