학술논문
香草醛法测定紫山药中原花青素的方法建立 / Established the Vanillin-HCl Method to Determine the Procyanidins in Purple Yam
Document Type
Academic Journal
Author
王彦平; 杨会会; 徐方延; 赵峥嵘; 古洋; 陈月英; WANG Yan-ping; YANG Hui-hui; XU Fang-yan; ZHAO Zheng-rong; GU Yang; CHEN Yue-ying
Source
食品研究与开发 / Food Research and Development. 38(15):152-155
Subject
Language
Chinese
ISSN
1005-6521
Abstract
建立香草醛-盐酸法测定紫山药中原花青素含量的方法.主要从光照影响、反应时间、反应温度、香草醛用量和盐酸浓度5个方面对试验条件进行优化,结果表明测定的最佳条件为:紫山药提取液1 mL,10 g/100 mL香草醛溶液5 mL,浓盐酸3mL,而后用甲醇定容至10 mL,在20℃下静置10 min,于500 nm处测定其吸光度.经测定该方法线性范围为4 μg/mL～24 μg/mL,相对标准偏差RSD为1.10％,回收率为98.06 ％～103.89％,可以作为紫山药中原花青素的测定方法.
The vanillin method to determine the concentration of procyanidins in purple yam was established.The experimental conditions were optimized from five aspects:effect of illumination,reaction time,reaction temperature,the amount of vanillin and concentration of hydrochloric acid.The experimental results showed that when 1 mL extracting solution of purple yam was injected to volumetric flask,the optimal reaction conditions were as the following:5 mL of 10 g/100 mL vanillin solution,3 mL of concentrated hydrochloric acid.After diluted to 10 mL,the volumetric flask was placed in 20 ℃ for 10 minutes,and then the absorbance was detected at 500 nm.According to the results,the line of range was from 4 μg/mL to 24 μg/mL,the relative standard deviation was 1.10 ％,and the recoveries were from 98.06 ％ to 103.89 ％.The results proved that this method could be used for the determination of proanthocyanidins in purple yam.
The vanillin method to determine the concentration of procyanidins in purple yam was established.The experimental conditions were optimized from five aspects:effect of illumination,reaction time,reaction temperature,the amount of vanillin and concentration of hydrochloric acid.The experimental results showed that when 1 mL extracting solution of purple yam was injected to volumetric flask,the optimal reaction conditions were as the following:5 mL of 10 g/100 mL vanillin solution,3 mL of concentrated hydrochloric acid.After diluted to 10 mL,the volumetric flask was placed in 20 ℃ for 10 minutes,and then the absorbance was detected at 500 nm.According to the results,the line of range was from 4 μg/mL to 24 μg/mL,the relative standard deviation was 1.10 ％,and the recoveries were from 98.06 ％ to 103.89 ％.The results proved that this method could be used for the determination of proanthocyanidins in purple yam.