학술논문
产前应激对情绪相关脑区c-Fos神经元激活的影响 / Impact of prenatal stress on evoked expression of c-Fos in emotion-related brain areas
Document Type
Academic Journal
Author
Source
首都医科大学学报 / Journal of Capital Medical University. 44(6):1014-1021
Subject
Language
Chinese
ISSN
1006-7795
Abstract
目的 探讨产前应激(prenatal stress,PS)对抑郁样行为子代大鼠情绪相关脑区c-Fos神经元激活的影响.方法 利用产前束缚应激建立抑郁样行为子代大鼠模型.应用免疫荧光染色方法检测正常对照组和PS组大鼠在内侧前额叶皮质(medial prefrontal cortex,mPFC)脑区、基底外侧杏仁核(basolateral amygdala,BLA)脑区、腹侧海马(ventral hippocampus,VH)脑区和腹外侧中脑导水管周围黑质(ventrolateral periaqueductal gray,vlPAG)脑区等情绪相关脑区的c-Fos与NeuN蛋白的表达并观察上述脑区应激敏感性神经元密度和比例的改变.结果 与对照组相比,PS组大鼠mPFC及BLA脑区c-Fos应激敏感性神经元密度和比例降低,VH脑区c-Fos应激敏感性神经元密度降低;vlPAG脑区 c-Fos应激敏感性神经元密度不变.结论 PS 导致子代大鼠mPFC、BLA及VH脑区c-Fos神经元激活密度下降,这种改变可能与抑郁样行为存在相关性.
Objective To elucidate the impact of prenatal stress(PS)on evoked expression of c-Fos in select emotion-related brain areas.Methods An experimental model of maternal exposure to chronic restraint stress in rats was employed as PS model.Subsequently,we assessed c-Fos and NeuN staining by immunofluorescence staining method in various brain regions of PS offspring rats,including the medial prefrontal cortex(mPFC),basolateral amygdala(BLA),ventral hippocampus(VH),and ventrolateral periaqueductal gray(vlPAG).Results Our findings revealed a significant decrease in the density and proportion of c-Fos positive neurons in the mPFC and BLA regions of PS-exposed offspring compared to control offspring.The density of c-Fos positive neurons in the VH region reduced in PS-exposed offspring,while no change was observed in the vlPAG region.Conclusion Our results indicated that PS induced a decreased density of stress-sensitive neurons in mPFC,BLA and VH brain regions,suggesting a potential underlying mechanism for the observed behavioral changes.
Objective To elucidate the impact of prenatal stress(PS)on evoked expression of c-Fos in select emotion-related brain areas.Methods An experimental model of maternal exposure to chronic restraint stress in rats was employed as PS model.Subsequently,we assessed c-Fos and NeuN staining by immunofluorescence staining method in various brain regions of PS offspring rats,including the medial prefrontal cortex(mPFC),basolateral amygdala(BLA),ventral hippocampus(VH),and ventrolateral periaqueductal gray(vlPAG).Results Our findings revealed a significant decrease in the density and proportion of c-Fos positive neurons in the mPFC and BLA regions of PS-exposed offspring compared to control offspring.The density of c-Fos positive neurons in the VH region reduced in PS-exposed offspring,while no change was observed in the vlPAG region.Conclusion Our results indicated that PS induced a decreased density of stress-sensitive neurons in mPFC,BLA and VH brain regions,suggesting a potential underlying mechanism for the observed behavioral changes.