학술논문
临床分离携带psm-mec基因耐甲氧西林表皮葡萄球菌的SCCmec型别分析 / Homologous SCCmec elements in clinical isolates of methicillin-resistant Staphylococcus epidermidis carrying psm-mec
Document Type
Academic Journal
Author
Source
西安交通大学学报（医学版） / Journal of Xi'an Jiaotong University(Medical Sciences). 38(2):257-260
Subject
Language
Chinese
ISSN
1671-8259
Abstract
目的 探讨临床分离携带psm-mec基因耐甲氧西林表皮葡萄球菌(MRSE)的SCCmec型别.方法 收集临床分离并经全自动微生物鉴定系统准确鉴定的表皮葡萄球菌165株,通过PCR扩增esp和mecA基因准确鉴定MRSE,扩增psm-mec、fudoh和p221片段鉴定携带psm-mec菌株.采用多重PCR对携带psm-mec基因的MRSE的mec、ccr和SCCmec进行分型.结果 138株MRSE菌株中,29株携带psm-mec基因,携带率为17.58%.多重PCR对mec和ccr分型结果显示,携带psm-mec基因MRSE均为Class A mec,但ccr型别存在明显的多样性.多重PCR对SCCmec分型结果显示,所有菌株均可扩增出Ⅱ型和/或Ⅲ型SCCmec条带,且为混合型别.结论 临床分离携带psm-mec基因的MRSE的SCCmec具有同源性,以含Class A mec的携带Ⅱ型和/或Ⅲ型SCCmec为主.
Objective To investigate SCCmec types in clinical isolates of methicillin-resistant Staphylococcus epidermidis (MRSE) carrying psm-mec.Methods We collected 165 strains of Staphylococcus epidermidis identified by automated microbiological identification system and screened MRSE by PCR amplification of esp and mecA gene.Strains with psm-mec were identified by amplification of psm-mec,fudoh and p221 DNA fragment;mec,ccr and SCCmec typing was conducted by multiplex PCR assay.Results Among 138 strains of MRSE,29 strains were identified as MRSE with psm-mec,and the carrying rate was 17.58%.Results of mec and ccr typing by multiple PCR showed that MRSE with psm-mec carried Class A mec,but the ccr type had obvious diversity.Results of SCCmec typing showed that all strains with psm-mec belonged to type Ⅱ and/or Ⅲ SCCmec.Conclusion Clinical isolates of MRSE with psm-mec carry homologous type Ⅱ and/or Ⅲ SCCmec harboring Class A mec.
Objective To investigate SCCmec types in clinical isolates of methicillin-resistant Staphylococcus epidermidis (MRSE) carrying psm-mec.Methods We collected 165 strains of Staphylococcus epidermidis identified by automated microbiological identification system and screened MRSE by PCR amplification of esp and mecA gene.Strains with psm-mec were identified by amplification of psm-mec,fudoh and p221 DNA fragment;mec,ccr and SCCmec typing was conducted by multiplex PCR assay.Results Among 138 strains of MRSE,29 strains were identified as MRSE with psm-mec,and the carrying rate was 17.58%.Results of mec and ccr typing by multiple PCR showed that MRSE with psm-mec carried Class A mec,but the ccr type had obvious diversity.Results of SCCmec typing showed that all strains with psm-mec belonged to type Ⅱ and/or Ⅲ SCCmec.Conclusion Clinical isolates of MRSE with psm-mec carry homologous type Ⅱ and/or Ⅲ SCCmec harboring Class A mec.