학술논문
3种中药成分对玉米赤霉烯酮致小鼠肝脏损伤的缓解作用 / Alleviative Effects of Three Traditional Chinese Medicine Components on Liver Injury in Zearalenone Poisoned Mice
Document Type
Academic Journal
Author
Source
中国畜牧兽医 / China Animal Husbandry & Veterinary Medicine. 51(1):434-442
Subject
Language
Chinese
ISSN
1671-7236
Abstract
[目的]探究中药对玉米赤霉烯酮(zearalenone,ZEA)致动物肝损伤的缓解作用.为临床研发新中兽药和饲料添加剂提供理论依据.[方法]将63只8周龄昆明雌鼠随机分为7组,每组9只.对照组,上、下午灌胃生理盐水各1次;ZEA组,上午灌胃20 mg/kg ZEA,下午灌胃生理盐水;槲皮素、山奈酚、芦丁和成分复方组为中药成分治疗组,上午灌胃20 mg/kg ZEA,下午分别灌胃50 mg/kg槲皮素、50 mg/kg山奈酚、100 mg/kg芦丁、100 mg/kg成分复方(V(槲皮素)∶V(山奈酚)∶V(芦丁)=1∶2∶2);阳性对照组,上午灌胃20 mg/kg ZEA,下午灌胃100 mg/kg维生素E;每只每次均灌胃0.2 mL,连续7 d.于第8天称量各组小鼠体重,眼球摘除采血,分离血清,脱颈处死小鼠,剥离肝脏并称重,计算肝脏指数,检测血清肝脏功能、抗氧化酶活性和肝脏炎症指标.[结果]生长发育检测结果显示,与对照组相比,ZEA组小鼠体重降低、肝脏重量升高(P＜0.05);与ZEA组相比,芦丁和成分复方组体重升高(P＜0.05).血清肝脏功能检测结果,与对照组相比,ZEA组血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)活性均升高(P＜0.05);与ZEA组相比,中药成分和维生素E治疗组各个酶活性均降低(P＜0.05).肝脏氧化应激检测结果显示,与对照组相比,ZEA组肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性、谷胱甘肽(GSH)含量和总抗氧化能力(T-AOC)均降低(P＜0.05),丙二醛(MDA)和LDH含量升高(P＜0.05);与ZEA组相比,中药成分和维生素E治疗组SOD、CAT活性、GSH含量和T-AOC均升高(P＜0.05),MDA和LDH含量降低(P＜0.05).炎症因子检测结果,与对照组相比,ZEA组机体肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)和IL-6含量升高、IL-10含量降低(P＜0.05);与ZEA组相比,中药成分和维生素E治疗组机体TNF-α、IL-1β和IL-6含量降低(P＜0.05),槲皮素和维生素E治疗组肝脏IL-10含量升高(P＜0.05).[结论]3种中药成分槲皮素、山奈酚、芦丁和维生素E均可缓解ZEA致小鼠肝脏的损伤,山奈酚效果最佳.
[Objective]This study was aimed to explore the relieving effect of traditional Chinese medicine on liver damage caused by zearalenone(ZEA)in animals.[Method]63 8-week-old Kunming female mice were randomly divided into 7 groups,with 9 mice in each group.In control group,physiological saline was administered orally once in the morning and once in the afternoon;ZEA group,20 mg/kg ZEA was gavaged in the morning and physiological saline was gavaged in the afternoon.Quercetin,kaempferol,rutin,and compound ingredients groups were traditional Chinese medicine treatment groups.In the morning,20 mg/kg ZEA was administered by gavage,and in the afternoon,50 mg/kg quercetin,50 mg/kg kaempferol,100 mg/kg rutin,and 100 mg/kg compound ingredients(V(quercetin)∶V(kaempferol)∶V(rutin)=1∶2∶2)were administered by gavage,respectively.Positive control group,20 mg/kg ZEA were administered by gavage in the morning and 100 mg/kg vitamin E was administered by gavage in the afternoon.Each animal was gavaged with 0.2 mL each time for 7 consecutive days.The mice were euthanized on the 8th day to detect body weight,liver index,serum liver function,antioxidant enzyme activity,and liver inflammation indicators.[Result]The weight development results showed that compared with control group,ZEA group mice had lower body weight and higher liver weight(P＜0.05).Compared with ZEA group,the weight of rutin and compound groups were increased(P＜0.05).Compared with control group,the serum liver function test results showed that the activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),acid phosphatase(ACP),lactate dehydrogenase(LDH)in ZEA group were increased(P＜0.05).Compared with ZEA group,the activity of various enzymes in traditional Chinese medicine and vitamin E treatment groups were decreased(P＜0.05).The results of liver oxidative stress test showed that compared with control group,the activity of superoxide dismutase(SOD),catalase(CAT),glutathione(GSH)content,and total antioxidant capacity(T-AOC)in liver of ZEA group were decreased(P＜0.05),while the content of malondialdehyde(MDA)and LDH were increased(P＜0.05).Compared with ZEA group,the activity of SOD,CAT,GSH content and T-AOC in traditional Chinese medicine and vitamin E treatment groups were increased(P＜0.05),while the content of MDA and LDH were decreased(P＜0.05).Inflammatory factor detection results showed that compared with control group,the content of tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1β)and IL-6 in ZEA group were increased and the content of IL-10 was decreased(P＜0.05).Compared with ZEA group,TNF-α,IL-1β and IL-6 contents of traditional Chinese medicine ingredient and vitamin E treatment groups were decreased(P＜0.05),while the IL-10 content were increased in quercetin and vitamin E treatment groups(P＜0.05).[Conclusion]All three traditional Chinese medicine ingredients quercetin,kaempferol,rutin and vitamin E could alleviate the liver damage caused by ZEA in mice,and the effect of kaempferol was the best.
[Objective]This study was aimed to explore the relieving effect of traditional Chinese medicine on liver damage caused by zearalenone(ZEA)in animals.[Method]63 8-week-old Kunming female mice were randomly divided into 7 groups,with 9 mice in each group.In control group,physiological saline was administered orally once in the morning and once in the afternoon;ZEA group,20 mg/kg ZEA was gavaged in the morning and physiological saline was gavaged in the afternoon.Quercetin,kaempferol,rutin,and compound ingredients groups were traditional Chinese medicine treatment groups.In the morning,20 mg/kg ZEA was administered by gavage,and in the afternoon,50 mg/kg quercetin,50 mg/kg kaempferol,100 mg/kg rutin,and 100 mg/kg compound ingredients(V(quercetin)∶V(kaempferol)∶V(rutin)=1∶2∶2)were administered by gavage,respectively.Positive control group,20 mg/kg ZEA were administered by gavage in the morning and 100 mg/kg vitamin E was administered by gavage in the afternoon.Each animal was gavaged with 0.2 mL each time for 7 consecutive days.The mice were euthanized on the 8th day to detect body weight,liver index,serum liver function,antioxidant enzyme activity,and liver inflammation indicators.[Result]The weight development results showed that compared with control group,ZEA group mice had lower body weight and higher liver weight(P＜0.05).Compared with ZEA group,the weight of rutin and compound groups were increased(P＜0.05).Compared with control group,the serum liver function test results showed that the activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),acid phosphatase(ACP),lactate dehydrogenase(LDH)in ZEA group were increased(P＜0.05).Compared with ZEA group,the activity of various enzymes in traditional Chinese medicine and vitamin E treatment groups were decreased(P＜0.05).The results of liver oxidative stress test showed that compared with control group,the activity of superoxide dismutase(SOD),catalase(CAT),glutathione(GSH)content,and total antioxidant capacity(T-AOC)in liver of ZEA group were decreased(P＜0.05),while the content of malondialdehyde(MDA)and LDH were increased(P＜0.05).Compared with ZEA group,the activity of SOD,CAT,GSH content and T-AOC in traditional Chinese medicine and vitamin E treatment groups were increased(P＜0.05),while the content of MDA and LDH were decreased(P＜0.05).Inflammatory factor detection results showed that compared with control group,the content of tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1β)and IL-6 in ZEA group were increased and the content of IL-10 was decreased(P＜0.05).Compared with ZEA group,TNF-α,IL-1β and IL-6 contents of traditional Chinese medicine ingredient and vitamin E treatment groups were decreased(P＜0.05),while the IL-10 content were increased in quercetin and vitamin E treatment groups(P＜0.05).[Conclusion]All three traditional Chinese medicine ingredients quercetin,kaempferol,rutin and vitamin E could alleviate the liver damage caused by ZEA in mice,and the effect of kaempferol was the best.