학술논문
健脾补肾法对人结直肠癌移植瘤组织TLR4、RAGE、NF-κB及CD34、D2-40的影响 / Effects of Spleen-and-Kidney Invigorating Method on TLR4,RAGE,NF-KB,CD34,D2-40 in Human Colorectal Cancer Transplanted Tumor Tissue
Document Type
Academic Journal
Author
王梦涵; 姚欣; 袁婉婧; 李梅; 黄兴瑜; 孙怡; 曾宏亮; 谢彪; WANG Meng-han; YAO Xin; YUAN Wan-jing; LI Mei; HUANG Xing-yu; SUN Yi; ZENG Hong-liang; XIE Biao
Source
云南中医中药杂志 / Yunnan Journal of Traditional Chinese Medicine and Materia Medica. 44(3):78-82
Subject
Language
Chinese
ISSN
1007-2349
Abstract
目的 探讨健脾补肾法对人结直肠癌移植瘤组织TLR4、RAGE、NF-KB及CD34、D2-40的影响.方法 于裸鼠右腿内侧接种人结肠癌细胞,建立人结肠癌裸鼠皮下移植瘤模型,15d后脱颈处死剥离瘤体.对照组用生理盐水灌胃,实验组用健脾补肾中药灌胃,免疫组化法检测2组移植瘤组织中TLR4、RAGE、NF-κB及CD34、D2-40的表达.结果 免疫组化显示,与对照组相比,实验组的移植瘤组织中TLR4、RAGE、NF-κB的表达均明显下调(P＜0.05),微血管CD34及淋巴管D2-40也明显下调(P＜0.005).结论 健脾补肾法能明显下调人结直肠癌移植瘤组织炎症受体TLR4及RAGE、核转录因子NF-κB、肿瘤新生血管标记物CD34及肿瘤新生淋巴管标记物D2-40的表达.