학술논문
两株从三带喙库蚊中分离的toti病毒变异株的分子特征 / MOLECULAR CHARACTERIZATION OF TWO VARIANT TOTIVIRUSES ISOLATED FROM CULEX TRITAENIORHYNCHUS IN CHINA
Document Type
Academic Journal
Author
彭红红; 王媛媛; 代科; 赵秋敏; 左曙青; 童贻刚; 张久松; PENG Hong-Hong; WANG Yuan-Yuan; DAI Ke; ZHAO Qiu-Min; ZUO Shu-Qing; TONG Yi-Gang; ZHANG Jiu-Song
Source
寄生虫与医学昆虫学报 / Acta Parasitology et Medica Entomologica Sinica. 27(1):20-25
Subject
Language
Chinese
ISSN
1005-0507
Abstract
从上海捕获的三带喙库蚊中新分离到2株toti病毒,暂命名为Toti病毒ND3(TOV_ND3)和Toti病毒FD17(TOV_FD17).利用高通量测序平台测定其全基因组序列.结果表明,TOV_ND3和FD17的基因组长度分别为7612和7611 bp,编码2个蛋白,包括1个衣壳蛋白(CP)和1个RNA依赖的RNA聚合酶(RdRp).TOV_ND3的全基因组序列与Culex tritaeniorhynchus NJ2(CTV_NJ2)的同源性最高,然而TOV_FD17与Omono river病毒AK4(ORV-AK4)的同源性最高,分别为84.6％和84.9％,两株之间核苷酸同源性为74.1％.系统发育分析表明,这两株新分离的病毒株属于Toti病毒科的变异株.
Two totiviruses, temporarily named Totivirus ND3 (TOV_ND3) and FD17 (TOV_FD17), were isolated from Culex tritaeniorhynchus captured in Shanghai City. The full sequences were determined using high-throughput sequencing method. The results showed that the genomes of TOV_ND3 and FD17 were 7612 and 7611 nucleotides, respectively in length, and encoded a capsid protein and an RNA-dependent RNA polymerase. The nucleotide sequence of TOV_ND3 had the highest similarity to Cx. tritaeniorhynchus totivirus NJ2, while the FD17 was most similar to Omono River virus strain AK4, with 84. 6％ and 84. 9％ identity, respectively, and 74. 1％ identity between themselves. Phylogenetic analysis revealed that the two newly obtained viruses were grouped into the unassigned totiviruses. Our results suggested that the two new viruses were variant totiviruses.
Two totiviruses, temporarily named Totivirus ND3 (TOV_ND3) and FD17 (TOV_FD17), were isolated from Culex tritaeniorhynchus captured in Shanghai City. The full sequences were determined using high-throughput sequencing method. The results showed that the genomes of TOV_ND3 and FD17 were 7612 and 7611 nucleotides, respectively in length, and encoded a capsid protein and an RNA-dependent RNA polymerase. The nucleotide sequence of TOV_ND3 had the highest similarity to Cx. tritaeniorhynchus totivirus NJ2, while the FD17 was most similar to Omono River virus strain AK4, with 84. 6％ and 84. 9％ identity, respectively, and 74. 1％ identity between themselves. Phylogenetic analysis revealed that the two newly obtained viruses were grouped into the unassigned totiviruses. Our results suggested that the two new viruses were variant totiviruses.