학술논문
广州地区不同检测系统精子浓度和精子活动力参考区间的建立及验证 / Establishment and verification for the reference intervals of sperm concentration and sperm total motility by different determination systems in Guangzhou
Document Type
Academic Journal
Author
Source
检验医学 / Laboratory Medicine. 33(1):10-14
Subject
Language
Chinese
ISSN
1673-8640
Abstract
目的 按不同地域人群及检测系统建立精液常规分析主要指标(精子浓度、精子活动力)的参考区间,并验证其是否适用于广州地区的人群.方法 根据美国临床实验室标准化协会(CLSI)C28-A3文件,分别采用3种不同的检测系统[计算机辅助精液分析系统(CASA)、Marker计数板、改良牛鲍计数板]检测145名体检健康男性的精子浓度和精子活动力并建立各自的参考区间,另收集20名健康男性个体,用于验证参考区间的适用性.结果 CASA、Marker计数板、改良牛鲍计数板检测精子浓度的参考区间分别为≥27.6×106/mL、≥35.4×106/mL、≥31.0×106/mL.CASA、Marker计数板测定精子总活动力的参考区间分别为≥40.9％、≥49.4％,前向活动力的参考区间分别为≥32.2％、≥35.0％.结论 建立的精子浓度和精子活动力参考区间适用于广州地区的人群,亦可推广到本地区内的其他实验室.
Objective To establish the reference intervals of semen routine analysis's main indicators (sperm concentration and sperm total motility)according to regional characteristics and different determination systems, and to verify whether they are suitable for Guangzhou local population. Methods According to the Clinical and Laboratory Standards Institute (CLSI) C28-A3 document,145 healthy subjects were enrolled. Computer assisted sperm analysis(CASA), Marker plate and improved NiuBao plate were used. The reference intervals of 3 different determination systems were established. Another 20 cases of healthy subjects were enrolled to verify the applicability of these reference intervals. Results The reference intervals for sperm concentration were ≥27.6×106/mL for CASA,≥35.4×106/mL for Marker plate and ≥31.0×106/mL for improved NiuBao plate. The reference intervals for sperm total motility were ≥40.9％ for CASA and ≥49.4％ for Marker plate. The reference intervals for sperm forward motility were ≥32.2％ for CASA and ≥35.0％ for Marker plate. Conclusions The established reference intervals are applicable for our clinical laboratory and local population,even they can be used for other clinical laboratories in local area.
Objective To establish the reference intervals of semen routine analysis's main indicators (sperm concentration and sperm total motility)according to regional characteristics and different determination systems, and to verify whether they are suitable for Guangzhou local population. Methods According to the Clinical and Laboratory Standards Institute (CLSI) C28-A3 document,145 healthy subjects were enrolled. Computer assisted sperm analysis(CASA), Marker plate and improved NiuBao plate were used. The reference intervals of 3 different determination systems were established. Another 20 cases of healthy subjects were enrolled to verify the applicability of these reference intervals. Results The reference intervals for sperm concentration were ≥27.6×106/mL for CASA,≥35.4×106/mL for Marker plate and ≥31.0×106/mL for improved NiuBao plate. The reference intervals for sperm total motility were ≥40.9％ for CASA and ≥49.4％ for Marker plate. The reference intervals for sperm forward motility were ≥32.2％ for CASA and ≥35.0％ for Marker plate. Conclusions The established reference intervals are applicable for our clinical laboratory and local population,even they can be used for other clinical laboratories in local area.