학술논문
多发性肌炎/皮肌炎患者血清中可溶性人类白细胞抗原-G表达水平的研究 / Analysis of serum soluble human leukocyte antigen-G levels in patients with polymyositis or dermatomyositis
Document Type
Academic Journal
Author
Source
中华风湿病学杂志 / Chinese Journal of Rheumatology. 17(5):313-317
Subject
Language
Chinese
ISSN
1007-7480
Abstract
目的 检测多发性肌炎/皮肌炎患者血清可溶性人类白细胞抗原-G (sHLA-G)水平,并分析其与多发性肌炎/皮肌炎各临床特征及治疗和预后的关系,探讨血清sHLA-G在多发性肌炎/皮肌炎发病机制中的可能作用.方法 利用酶联免疫吸附试验(ELISA)方法测定96例多发性肌炎/皮肌炎患者(其中多发性肌炎26例,皮肌炎70例)和35名健康体检者血清sHLA-G水平,分析血清sHLA-G与多发性肌炎/皮肌炎患者临床特征、实验室指标及治疗、预后的关系.结果 血清sHLA-G水平在多发性肌炎/皮肌炎患者[(44±70) U/ml]明显高于健康对照者[(4±5)U/ml,P＜0.01)].皮肌炎患者组血清sHLA-G水平[(54±81) U/ml]明显高于多发性肌炎患者组[(27±41) U/ml,P=0.004].血清sHLA-G升高组多发性肌炎/皮肌炎患者中吞咽困难的发生率较sHLA-G正常组明显升高(P=0.001).sHLA-G水平与CD3+T细胞和CD4+T细胞水平均呈负相关(r=-0.233,P=0.047；r=-0.287,P=0.015),与补体C3水平呈正相关(r=0.284,P=0.021).初治组患者血清sHLA-G水平[(77±99) U/ml]显著高于经治组[(34±52) U/ml,P=0.021].但血清sHLA-G水平与疾病活动度无明显相关,糖皮质激素及免疫抑制剂治疗对血清sHLA-G水平也无明显影响.结论 血清sHLA-G水平在多发性肌炎/皮肌炎患者中明显升高,并且与外周血CD3+和CD4+T细胞数呈负相关,临床上与吞咽困难的发生相关,提示sHLA-G可能在多发性肌炎/皮肌炎病理过程中发挥一定作用.
Objective To investigate the serum levels of soluble human leukocyte antigen (sHLA)-G in patients with polymyositis (PM) or dermatomyositis (DM),and to analyze its association with clinical features and possible role in the pathogenesis of PM/DM.Methods Serum sHLA-G levels of 26 patients with PM,70 patients with DM and 35 matched healthy controls were measured by ELISA.The relationship between the sHLA-G levels and clinical features or seroimmunological data in the patients with PM/DM was analyzed.Results Serum levels of sHLA-G in PM/DM patients were significantly higher compared to healthy controls [(44±70) U/ml vs (4±5) U/ml,P＜0.01].There was statistically significant difference between DM patients and PM patients [(54±81) U/ml vs (27±41) U/ml,P=0.004].The incidence of dysphagia was significantly higher in sHLA-G elevated group than those in sHLA-G normal group (P=0.001).Additionally,Spearman rank correlation analysis showed that the serum sHLA-G levels were positively correlated with serum C3 (r=0.284,P=0.021),but negatively correlated with CD3+ T cells (r=-0.233,P=0.047) and CD4+ T cells (r=-0.287,P=0.015) in the peripheral blood in patients with PM/DM.Serum levels of sHLA-G in non-treated PM/DM patients were significantly higher compared to treated patients [(77±99) U/ml vs (34±52) U/ml,P=0.021].No relationship between serum sHLA-G levels and PM/DM disease activity,or different drug therapy was found.Conclusion Serum levels of sHLA-G are increased in PM/DM patients.The increased production of sHLA-G,paralleled with higher incidence of dysphagia and lower level of CD3+ T cells and CD4+ T cells,indicates that sHLA-G may play an important role in the pathogenesis of PM/DM.
Objective To investigate the serum levels of soluble human leukocyte antigen (sHLA)-G in patients with polymyositis (PM) or dermatomyositis (DM),and to analyze its association with clinical features and possible role in the pathogenesis of PM/DM.Methods Serum sHLA-G levels of 26 patients with PM,70 patients with DM and 35 matched healthy controls were measured by ELISA.The relationship between the sHLA-G levels and clinical features or seroimmunological data in the patients with PM/DM was analyzed.Results Serum levels of sHLA-G in PM/DM patients were significantly higher compared to healthy controls [(44±70) U/ml vs (4±5) U/ml,P＜0.01].There was statistically significant difference between DM patients and PM patients [(54±81) U/ml vs (27±41) U/ml,P=0.004].The incidence of dysphagia was significantly higher in sHLA-G elevated group than those in sHLA-G normal group (P=0.001).Additionally,Spearman rank correlation analysis showed that the serum sHLA-G levels were positively correlated with serum C3 (r=0.284,P=0.021),but negatively correlated with CD3+ T cells (r=-0.233,P=0.047) and CD4+ T cells (r=-0.287,P=0.015) in the peripheral blood in patients with PM/DM.Serum levels of sHLA-G in non-treated PM/DM patients were significantly higher compared to treated patients [(77±99) U/ml vs (34±52) U/ml,P=0.021].No relationship between serum sHLA-G levels and PM/DM disease activity,or different drug therapy was found.Conclusion Serum levels of sHLA-G are increased in PM/DM patients.The increased production of sHLA-G,paralleled with higher incidence of dysphagia and lower level of CD3+ T cells and CD4+ T cells,indicates that sHLA-G may play an important role in the pathogenesis of PM/DM.