학술논문
生长激素对重症急性胰腺炎肝脏损伤的保护作用 / Protection of recombinant human growth hormone on rat liver with severe acute pancreatitis
Document Type
Academic Journal
Author
钟朝辉; 周迈; 王达庆; 焦岗军; 邓五一; 冷希圣; Zhong Zhaohui; Zhou Mai; Wang Daqing; Jiao Gangjun; Deng Wuyi; Leng Xisheng
Source
中华普通外科杂志 / Chinese Journal of General Surgery. 31(2):121-125
Subject
Language
Chinese
ISSN
1007-631X
Abstract
目的 观察重组人生长激素(rhGH)预处理对重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠肝脏损伤的作用. 方法 采用胰管注射牛磺胆酸钠制作大鼠SAP模型,40只雄性SD大鼠随机分为实验组(n=20)和对照组(n=20),另取20只雄性SD大鼠仅胰管注射盐水作为阴性对照组.实验组术前连续3d皮下注射rhGH,1 U·kg-1·d-1,对照组给予同体积生理盐水作为对照.分别在12h,24 h后检测血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素1β(interleukin-1β,IL-1β)水平及肝组织匀浆丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、血浆内毒素(Endotoxin)和D-乳酸(D-lactate)水平.同时比较各组间的肝细胞凋亡程度和胰腺病理评分.结果 与阴性对照组相比,对照组、实验组ALT、AST、TNF-α、IL-1β、MDA、内毒素和D--酸水平、肝细胞凋亡指数和胰腺组织病理评分在术后12 h和24 h明显增高(P＜0.05).实验组ALT、AST、TNF-α、内毒素和D-乳酸水平在术后12h和24 h均低于对照组(P＜0.05),而血清IL-1β水平在两组之间差异无统计学意义.实验组MDA水平在术后12h和24 h均明显低于对照组(P＜0.05).而SOD水平在各组之间差异无统计学意义.对照组肝细胞凋亡指数明显高于实验组(P=0.003),而实验组和对照组的胰腺组织病理评分差异无统计学意义.结论 重组人生长激素预处理对大鼠重症急性胰腺炎导致的肝脏损伤具有保护作用.
Objective To investigate the effect of recombinant human growth hormone (rhGH) on rat liver injury with severe acute pancreatitis.Methods Severe acute pancreatitis model was established by injection of sodium taurocholate into the pancreatobiliary duct in 40 male Sprague-Dawley rats.Rats were randomly divided into experiment group (n =20) and control group (n =20).Another 20 male SD rats injected saline served as negative control group.The experiment group were treated with subcutaneously injected rhGH for 3 days,1 U·kg-1 ·d-1.12 h and 24 h after operation,the level of ALT,AST,TNF-α,IL-1 β,SOD,MDA,endotoxin and D-lactate was detected respectively;the degree of live cell apoptosis and pathological score of pancreatic tissue were compared among these groups.Results In comparison with negative control group,the level of ALT,AST,TNF-α,IL-1β,MDA,endotoxin and D-lactate,the liver cell apoptosis index and pathological score of pancreatic tissuc were significantly higher in the control group and experiment group at 12 h and 24 h after operation (P ＜ 0.05).ALT,AST,TNF-α,endotoxin and D-lactate at 12 h and 24 h were significantly lower in the experiment group (P ＜ 0.05).MDA level significantly declined at 12 h and 24 h after operation in experiment group(P ＜ 0.05).Liver cell apoptosis index of the control group was higher than experiment group (P =0.003).Conclusion rhGH pretreatment relieves liver injury in rat with severe acute pancreatitis.
Objective To investigate the effect of recombinant human growth hormone (rhGH) on rat liver injury with severe acute pancreatitis.Methods Severe acute pancreatitis model was established by injection of sodium taurocholate into the pancreatobiliary duct in 40 male Sprague-Dawley rats.Rats were randomly divided into experiment group (n =20) and control group (n =20).Another 20 male SD rats injected saline served as negative control group.The experiment group were treated with subcutaneously injected rhGH for 3 days,1 U·kg-1 ·d-1.12 h and 24 h after operation,the level of ALT,AST,TNF-α,IL-1 β,SOD,MDA,endotoxin and D-lactate was detected respectively;the degree of live cell apoptosis and pathological score of pancreatic tissue were compared among these groups.Results In comparison with negative control group,the level of ALT,AST,TNF-α,IL-1β,MDA,endotoxin and D-lactate,the liver cell apoptosis index and pathological score of pancreatic tissuc were significantly higher in the control group and experiment group at 12 h and 24 h after operation (P ＜ 0.05).ALT,AST,TNF-α,endotoxin and D-lactate at 12 h and 24 h were significantly lower in the experiment group (P ＜ 0.05).MDA level significantly declined at 12 h and 24 h after operation in experiment group(P ＜ 0.05).Liver cell apoptosis index of the control group was higher than experiment group (P =0.003).Conclusion rhGH pretreatment relieves liver injury in rat with severe acute pancreatitis.