학술논문
黄秋葵发酵液对肉种鸡生产性能、肠道屏障及微生物组成的影响 / Effects of Adding Okra Fermentation Broth to Feed on Production Performance,Intestinal Barrier and Microbiota Composition in Broiler Breeder
Document Type
Academic Journal
Author
郭良兴; 聂芙蓉; 穆钊坤; 李志超; 孙青云; 魏甜甜; 全露露; 马铮财; GUO Liangxing; NIE Furong; MU Zhaokun; LI Zhichao; SUN Qingyun; WEI Tiantian; QUAN Lulu; MA Zhengcai
Source
中国家禽 / China Poultry. 46(2):36-43
Subject
Language
Chinese
ISSN
1004-6364
Abstract
试验旨在研究黄秋葵发酵液对肉种鸡生产性能、肠道屏障和微生物组成的影响.试验选取190日龄爱拔益加(AA)白羽肉种鸡40只随机分为对照组和黄秋葵发酵液(饲粮添加1%黄秋葵发酵液),每组5个重复,每个重复4只.试验期为49 d,其中预试期7 d,正试期42 d.试验结束后,收集盲肠组织和肠道内容物采用RT-qRCR检测肠道屏障相关基因(ZO-1、Claudin-1和Occludin)表达水平,ELISA检测肠道内容物中细胞因子含量,并且对肠道微生物细菌16S rRNA基因V3-V4区进行高通量测序以检测盲肠中微生物的组成.结果显示:饲粮添加黄秋葵发酵液显著提高白羽肉种鸡平均蛋重、日产蛋率和双黄蛋率(P<0.05),但对料蛋比和平均日增重没有显著影响(P>0.05);与对照组相比,FOP组白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)含量显著降低(P<0.05),白细胞介素-4(IL-4)和白细胞介素-10(IL-10)含量显著升高(P<0.05或P<0.01),肠道ZO-1、Claudin-1、Occludin基因表达水平显著上调(P<0.05).物种注释结果发现,在门水平,厚壁菌门(Firmicutes)和拟杆菌门(Bacterodietes)占比最高,与对照组相比,黄秋葵发酵液组厚壁菌门相对丰度升高(P<0.05),而拟杆菌门相对丰度下降(P<0.05);在属水平,黄秋葵发酵液组乳杆菌属(Lactobacillus)和梭杆菌属(Fusobacterium)相对丰度升高(P<0.05或P<0.01),拟杆菌属(Bacteroides)和罗姆布茨菌属(Romboutsia)相对丰度降低(P<0.05或P<0.01).KEGG富集通路分析表明,与对照组相比,黄秋葵发酵液组显著上调的通路主要涉及到抗生素的生物合成与调节机制、胆汁酸的生物合成等,显著下调的通路涉及到细胞凋亡、鞭毛组装以及细菌的趋化性等.研究表明,在本试验条件下,饲粮添加1%黄秋葵发酵液能够显著提高白羽肉种鸡平均蛋重、产蛋率和双黄蛋率,改变肠道内容物微生物区系组成,提升肠道屏障功能,改善肠道免疫微环境.
This experiment was conducted to study the effect of dietary okra fermentation broth on production performance,intestinal barrier and microbiota composition in broilers.Forty Arbor Acres(AA)white-feather broiler breeders aged 190 days were randomly divided into two groups,named as the control group and okra fermentation broth group(add 1% okra fermentation liquid to the diet),which was set five replicates in each group and four replicates in each group.The trial period was 49 days,in-cluding 7 days of pre-feeding period and 42 days of trial period.After the experiment,cecal tissue and intestinal contents were collected,RT-qRCR was used to detect the expression level of intestinal barrier related genes(ZO-1,Claudin-1 and Occludin),ELISA was used to detect the content of cytokines in intestinal contents,and high-throughput sequencing was performed on the V3-V4 region of intestinal microbial 16S rRNA gene to detect the composition of microorganisms in the cecum.The results showed that the addition of fermented okra extract to the feed significantly increased the average egg weight,daily egg production rate and double yolk egg rate of white-feather broiler breeder(P<0.05),but had no significant effect on the feed to egg ratio and average daily gain(P>0.05).Compared with the control group,okra fermentation broth group showed a significant decrease in the levels of interleukin-6(IL-6)and interleukin-1β(IL-1β)(P<0.05),and a significant increase in the levels of interleukin-4(IL-4)and interleukin-10(IL-10)(P<0.05 or P<0.01),the expression levels of ZO-1,Claudin-1 and Occludin genes of which were sig-nificantly increased(P<0.05).The results of species annotation showed that at the phylum level,compared with the control group,the relative abundance of Firmicutes in okra fermentation broth group was increased(P<0.05),while the relative abundance of Bacteroides was decreased(P<0.05);At the genus level,the relative abundances of Lactobacillus and Fusobacterium in FOP group were increased(P<0.05 or P<0.01),while the relative abundances of Bacteroides and Rombousia were decreased(P<0.05 or P<0.01);The analysis of KEGG enrichment pathway showed that compared with the control group,the significantly up-regulated pathway in okra fermentation broth group mainly involved the biosynthesis and regulation mechanism of antibiotics and the bio-synthesis of bile acid,while the significantly down-regulated pathway involved cell apoptosis,flagella assembly and bacterial che-motaxis(P<0.05 or P<0.01).In conclusion,under the experimental conditions,adding 1% okra fermentation broth to the diet could significantly increase the average egg weight,egg production rate and double yolk egg rate,change the microflora composi-tion of intestinal contents,improve the intestinal barrier function and improve the intestinal immune microenvironment of white-feather broiler breeder.
This experiment was conducted to study the effect of dietary okra fermentation broth on production performance,intestinal barrier and microbiota composition in broilers.Forty Arbor Acres(AA)white-feather broiler breeders aged 190 days were randomly divided into two groups,named as the control group and okra fermentation broth group(add 1% okra fermentation liquid to the diet),which was set five replicates in each group and four replicates in each group.The trial period was 49 days,in-cluding 7 days of pre-feeding period and 42 days of trial period.After the experiment,cecal tissue and intestinal contents were collected,RT-qRCR was used to detect the expression level of intestinal barrier related genes(ZO-1,Claudin-1 and Occludin),ELISA was used to detect the content of cytokines in intestinal contents,and high-throughput sequencing was performed on the V3-V4 region of intestinal microbial 16S rRNA gene to detect the composition of microorganisms in the cecum.The results showed that the addition of fermented okra extract to the feed significantly increased the average egg weight,daily egg production rate and double yolk egg rate of white-feather broiler breeder(P<0.05),but had no significant effect on the feed to egg ratio and average daily gain(P>0.05).Compared with the control group,okra fermentation broth group showed a significant decrease in the levels of interleukin-6(IL-6)and interleukin-1β(IL-1β)(P<0.05),and a significant increase in the levels of interleukin-4(IL-4)and interleukin-10(IL-10)(P<0.05 or P<0.01),the expression levels of ZO-1,Claudin-1 and Occludin genes of which were sig-nificantly increased(P<0.05).The results of species annotation showed that at the phylum level,compared with the control group,the relative abundance of Firmicutes in okra fermentation broth group was increased(P<0.05),while the relative abundance of Bacteroides was decreased(P<0.05);At the genus level,the relative abundances of Lactobacillus and Fusobacterium in FOP group were increased(P<0.05 or P<0.01),while the relative abundances of Bacteroides and Rombousia were decreased(P<0.05 or P<0.01);The analysis of KEGG enrichment pathway showed that compared with the control group,the significantly up-regulated pathway in okra fermentation broth group mainly involved the biosynthesis and regulation mechanism of antibiotics and the bio-synthesis of bile acid,while the significantly down-regulated pathway involved cell apoptosis,flagella assembly and bacterial che-motaxis(P<0.05 or P<0.01).In conclusion,under the experimental conditions,adding 1% okra fermentation broth to the diet could significantly increase the average egg weight,egg production rate and double yolk egg rate,change the microflora composi-tion of intestinal contents,improve the intestinal barrier function and improve the intestinal immune microenvironment of white-feather broiler breeder.