부산대학교 도서관

A reliable in vitro regeneration procedure for Populus tomentosa is a prerequisite for its trait improvement through genetic transformation.We established a systematic protocol for indirect regeneration of P.tomentosa using in vitro petioles of Chinese poplar cultivar ‘fasta-3'.A high frequency of callus induction (＞97 ％) was obtained from isolated petioles cultured on the modified 1/2MS basal medium supplemented with 0.5 mg/L ZT and 1.0 mg/L NAA,and the tested calli were subsequently plated on 1/2MS basal medium supplemented with 0.25 mg/L BA,0.25 mg/L ZT,0.25 mg/L NAA,0.01 mg/L TDZ,and 0.5 mg/L KT for efficient regeneration of shoots after being cultured for 6 weeks.The regenerated shoots were vigorously rooted on the tested media supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA.These results can facilitate genetic transformation of P.tomentosa for trait improvements in future.