학술논문
猫血浆对称性二甲基精氨酸参考范围的建立及其影响因素 / Establishment of Reference Range for Symmetric Dimethylarginine in Cat Plasma and Its Influencing Factors
Document Type
Academic Journal
Author
Source
中国兽医杂志 / Chinese Journal of Veterinary Medicine. 59(12):107-111
Subject
Language
Chinese
ISSN
0529-6005
Abstract
为了建立猫血浆对称性二甲基精氨酸(SDMA)的参考范围,并探索SDMA对猫慢性肾病(CKD)的诊断意义和临床应用价值,本试验从中国农业大学教学动物医院采集健康成年猫和慢性肾病(CKD)、心脏病(HD)、肿瘤、糖尿病(DM)、脂肪肝(FHL)和猫传染性腹膜炎(FIP)患猫的血浆样本,应用ELISA检测SDMA水平,建立健康成年猫的SDMA参考范围,并分析不同疾病对SDMA水平的影响.结果显示,健康成年猫血浆SDMA的参考范围为0～13.09 μg/dL,且不受年龄、性别和品种的影响(P＞0.05).CKD患猫血浆SDMA水平随着病情进展逐渐升高,且与肌酐(CREA)呈强相关性(r=0.890,P＜0.001),可反映肾功能的损伤程度.与健康成年对照组相比,FHL组和FIP组患猫血浆SDMA水平差异极显著(P＜0.01),HD组差异显著(P＜0.05),肿瘤组和DM组差异不显著(P＞0.05).结果表明,血浆SDMA水平对猫CKD早期诊断具有一定意义,猫脂肪肝和传染性腹膜炎可能会影响SDMA水平.
In order to establish the reference range of symmetric dimethylarginine(SDMA)in cat plasma and explore the diagnostic significance and clinical application value of SDMA in feline chronic kidney disease(CKD),this study collected plasma samples from healthy adult cats and cats with chronic kidney disease(CKD),heart disease(HD),tumors,diabetes mellitus(DM),feline hepatic lipidosis(FHL),and feline infectious peritonitis(FIP)from the China Agricultural University Veterinary Teaching Hospital.SDMA levels were measured using enzyme-linked immunosorbent assay(ELISA),and the reference range for SDMA in healthy adult cats was established.The influence of different diseases on SDMA levels was analyzed.The results showed that the reference range of plasma SDMA in healthy adult cats was 0 to 13.09 μg/dL,and it was not influenced by age,gender,and breed(P＞0.05).In cats with CKD,plasma SDMA levels gradually increased with the progression of the disease and were strongly correlation with creatinine(CREA)(r=0.890,P＜0.001),reflecting the degree of renal function damage.Compared with the healthy adult control group,cats in the FHL group and FIP group showed extremely significant differences in plasma SDMA levels(P＜0.01),and the HD group had a significant difference(P＜0.05),while the tumor group and DM group showed no significant difference(P＞0.05).The results indicate that plasma SDMA levels have certain significance for the early diagnosis of CKD in cats,and feline hepatic lipidosis and infectious peritonitis in cats may affect SDMA levels.
In order to establish the reference range of symmetric dimethylarginine(SDMA)in cat plasma and explore the diagnostic significance and clinical application value of SDMA in feline chronic kidney disease(CKD),this study collected plasma samples from healthy adult cats and cats with chronic kidney disease(CKD),heart disease(HD),tumors,diabetes mellitus(DM),feline hepatic lipidosis(FHL),and feline infectious peritonitis(FIP)from the China Agricultural University Veterinary Teaching Hospital.SDMA levels were measured using enzyme-linked immunosorbent assay(ELISA),and the reference range for SDMA in healthy adult cats was established.The influence of different diseases on SDMA levels was analyzed.The results showed that the reference range of plasma SDMA in healthy adult cats was 0 to 13.09 μg/dL,and it was not influenced by age,gender,and breed(P＞0.05).In cats with CKD,plasma SDMA levels gradually increased with the progression of the disease and were strongly correlation with creatinine(CREA)(r=0.890,P＜0.001),reflecting the degree of renal function damage.Compared with the healthy adult control group,cats in the FHL group and FIP group showed extremely significant differences in plasma SDMA levels(P＜0.01),and the HD group had a significant difference(P＜0.05),while the tumor group and DM group showed no significant difference(P＞0.05).The results indicate that plasma SDMA levels have certain significance for the early diagnosis of CKD in cats,and feline hepatic lipidosis and infectious peritonitis in cats may affect SDMA levels.