학술논문
甘肃冬小麦品种（系）面筋强度、黄色素含量和PPO活性相关基因的分子检测 / Molecular detection of genes associated with gluten strength, yellow pigment content and PPO activity of winter wheat cultivars (lines)in Gansu Province
Document Type
Academic Journal
Author
周喜旺; 宋建荣; 岳维云; 张耀辉; 曹世勤; 吕莉莉; 刘鸿燕; 王娜; 南海; 赵尚文; ZHOU Xi-wang; SONG Jian-rong; YUE Wei-yun; ZHANG Yao-hui; CAO Shi-qin; LYU Li-li; LIU Hong-yan; WANG Na; NAN Hai; ZHAO Shang-wen
Source
甘肃农业大学学报 / Journal of Gansu Agricultural University. (3):54-60
Subject
Language
Chinese
ISSN
1003-4315
Abstract
为了明确甘肃冬小麦品种(系)中品质相关基因的分布状况,提高品质育种效率,以141份小麦品种(系)为材料,利用高分子量麦谷蛋白亚基1Dx5的特异 PCR 标记、与黄色素含量相关的八氢番茄红素合酶(phytoene synthase,Psy)基因 Psy-A1的标记 YP7A 及多酚氧化酶(polyphenol oxidase,PPO)活性基因 Ppo-A1的标记PPO18分别进行1Dx5、Psy-A1和 Ppo-A1的基因型检测.结果表明：含1Dx5基因的材料56份,分布频率为39.7%；Psy-A1位点存在Psy-A1a和Psy-A1b 2种等位变异类型,分布频率分别为62.4%和37.6%；Ppo-A1位点存在Ppo-A1a和Ppo-A1b 2种等位变异类型,分布频率分别为42.6%和57.4%.1Dx5、Psy-A1和Ppo-A1的基因类型在不同地区分布频率存在明显差异,1Dx5和Psy-A1b基因在天水地区频率最高,分别为51.0%和40.8%；Ppo-A1b基因在庆阳地区频率最高,为77.8%,Psy-A1a基因在3地区的分布频率明显高于Psy-A1b基因.参试材料中,聚合1Dx5、Psy-A1b和Ppo-A1b基因的材料有19份,这些材料可作亲本资源,在小麦品种面筋强度和面粉色泽的改良中加以利用.
In order to define the distribution of genes related to quality traits and improve breeding effi-ciency,3 gene specific markers for HMW-GS 1Dx5 ,Psy-A1 and Ppo-A1 genes were used to detect the al-lelic variations at these loci in 141 Gansu wheat cultivars and advanced lines.The results indicated that the frequency of 1Dx5 gene was 39.7%,the frequencies of Psy-A1a genotype with high yellow pigment content and Psy-A1b genotype with low yellow pigment content at Psy-A1 locus were 62.4% and 37.6%,respec-tively;the frequencies of Ppo-A1 a genotype with high PPO activity and Ppo-A1 b genotype with low PPO activity at Ppo-A1 locus were 42.6% and 57.4%,respectively.Significant differences among 1Dx5,Psy-A1 and Ppo-A1 were found in different areas,1Dx5 and Psy-A1b were distributed mainly in Tianshui area, with frequency of 51.0% and 40.8%,respectively;Ppo-A1b had highest frequency of 77.8% in Qingyang area.The frequency of Psy-A1 a was obviously higher than that of Psy-A1 b in three areas.1 9 materials con-tented desired gene for 1Dx5 and alleles for Psy-A1b and Ppo-A1b,thus it is essential to combine the de-sired genes in a variety to improve the processing quality of Gansu winter wheat.The markers used in this study were all gene-specific,with good stability and high accuracy,and they can be efficiently applied in molecular marker-assisted breeding for wheat quality improvement.
In order to define the distribution of genes related to quality traits and improve breeding effi-ciency,3 gene specific markers for HMW-GS 1Dx5 ,Psy-A1 and Ppo-A1 genes were used to detect the al-lelic variations at these loci in 141 Gansu wheat cultivars and advanced lines.The results indicated that the frequency of 1Dx5 gene was 39.7%,the frequencies of Psy-A1a genotype with high yellow pigment content and Psy-A1b genotype with low yellow pigment content at Psy-A1 locus were 62.4% and 37.6%,respec-tively;the frequencies of Ppo-A1 a genotype with high PPO activity and Ppo-A1 b genotype with low PPO activity at Ppo-A1 locus were 42.6% and 57.4%,respectively.Significant differences among 1Dx5,Psy-A1 and Ppo-A1 were found in different areas,1Dx5 and Psy-A1b were distributed mainly in Tianshui area, with frequency of 51.0% and 40.8%,respectively;Ppo-A1b had highest frequency of 77.8% in Qingyang area.The frequency of Psy-A1 a was obviously higher than that of Psy-A1 b in three areas.1 9 materials con-tented desired gene for 1Dx5 and alleles for Psy-A1b and Ppo-A1b,thus it is essential to combine the de-sired genes in a variety to improve the processing quality of Gansu winter wheat.The markers used in this study were all gene-specific,with good stability and high accuracy,and they can be efficiently applied in molecular marker-assisted breeding for wheat quality improvement.