학술논문
天津滨海地区脑梗死与血管紧张素转化酶多态性相关性研究 / The association between cerebral infarction and angiotensin converting enzyme gene polymorphism in Binhai area Tianjin
Document Type
Academic Journal
Author
刘津; 刘运德; 李雪; 王彤宇; 李海平; 鲍会静; 李志军; Liu Jin; LiuYunde; Li Xue; Wang Tongyu; Li Haiping; Bao Huijing; Li Zhijun
Source
国际检验医学杂志 / International Journal of Laboratory Medicine. (15):2142-2144
Subject
Language
Chinese
ISSN
1673-4130
Abstract
目的：探讨天津滨海地区脑梗死发生与血管紧张素转化酶（ACE）多态性位点 rs4646994和rs35397082基因多态性的相关性。方法采用基因测序及DNA 电泳技术分别检测53例脑梗死患者和53例健康志愿者的 ACE的单核苷酸多态性（SNP）。同时收集各组血清样本，行血清 ACE检测。结果健康对照组和脑梗死患者组，rs35397082均为插入缺失型。对于rs4646994，健康对照组中rs4646994的插入型 SNP有45例（84．91％），缺失型为8例（15．09％）；脑梗死患者组中插入型47例（88．68％），缺失型为6例（11．32％），差异无统计学意义（P＞0．05）。健康对照组血清ACE水平与脑梗死患者组比较差异有统计学意义（P＜0．05），脑梗死患者组低于健康对照组。结论 ACE基因rs4646994和rs35397082多态性与天津滨海地区健康人及急性脑梗死无显著关联，二者血清水平差异不是由这两个位点差异所致，ACE基因的这两种SNP不是天津滨海地区急性脑梗死发生的独立危险因子。
Objective To study the association between the cerebral infarction and the angiotensin converting enzyme (ACE) gene rs4646994 and rs35397082 polymorphisms in Binhai area ,Tianjin .Methods Gene sequencing and DNA electrophoresis were used for the detection of the ACE gene single nucleotide polymorphisms (SNPs)(rs4646994 and rs35397082) .53 samples from pa‐tients with acute cerebral infarction and 53 samples from healthy volunteers were used in our study .Serum sample were collected from each group and tested by ACE ELISA .Results There were only deletion type of rs35397082 SNP in both of the control and cerebral infarction group .In the control group ,the number of insertion type of rs4646994 was 45(84 .91% ) ,deletion type was 8(15 . 09% ) and in the patients group ,the number of insertion was 47(88 .68% ) and the deletion was 6(11 .32% ) .There was no signifi‐cant difference between the patients group and the healthy donors (P>0 .05) .The concentration of ACE in control group was high‐er than the patients with acute cerebral infraction (P<0 .05) .Conclusion There is no significant association between the ACE gene polymorphisms(rs35397082 and rs4646994) and cerebral infarction in Binhai area ,Tianjin .The different concentration of ACE is not caused by these two SNPs .In this study ,these two SNPs are not the are not the risk factors of the cerebral infarction in Tianjin based on our study .
Objective To study the association between the cerebral infarction and the angiotensin converting enzyme (ACE) gene rs4646994 and rs35397082 polymorphisms in Binhai area ,Tianjin .Methods Gene sequencing and DNA electrophoresis were used for the detection of the ACE gene single nucleotide polymorphisms (SNPs)(rs4646994 and rs35397082) .53 samples from pa‐tients with acute cerebral infarction and 53 samples from healthy volunteers were used in our study .Serum sample were collected from each group and tested by ACE ELISA .Results There were only deletion type of rs35397082 SNP in both of the control and cerebral infarction group .In the control group ,the number of insertion type of rs4646994 was 45(84 .91% ) ,deletion type was 8(15 . 09% ) and in the patients group ,the number of insertion was 47(88 .68% ) and the deletion was 6(11 .32% ) .There was no signifi‐cant difference between the patients group and the healthy donors (P>0 .05) .The concentration of ACE in control group was high‐er than the patients with acute cerebral infraction (P<0 .05) .Conclusion There is no significant association between the ACE gene polymorphisms(rs35397082 and rs4646994) and cerebral infarction in Binhai area ,Tianjin .The different concentration of ACE is not caused by these two SNPs .In this study ,these two SNPs are not the are not the risk factors of the cerebral infarction in Tianjin based on our study .