학술논문
Spathaspora passalidarum突变株U-30木糖乙醇发酵条件研究 / Xylose-ethanol Fermentation Conditions for Spathaspora Passalidarum Mutant U-30
Document Type
Academic Journal
Author
黄文连; 陈叶福; 付更新; 杨金龙; 郭学武; 肖冬光; HUANG Wenlian; CHEN Yefu; FU Gengxin; YANG Jinlong; GUO Xuewu; XIAO Dongguang
Source
酿酒科技 / Liquor-Making Science & Technology. (11):49-53
Subject
Language
Chinese
ISSN
1001-9286
Abstract
Spathaspora passalidarum是近来发现的一种能天然利用木糖产乙醇的酵母菌,其发酵木糖速度比葡萄糖快,而且能混合发酵木糖、葡萄糖和纤维二糖.主要从氮源种类及浓度、接种量、转速及装液量、初始pH值等方面对Spathaspora passalidarum突变株U-30木糖乙醇发酵进行了研究,得到的优化摇瓶培养基组成为:初始木糖浓度为100 g/L,氮源为15 g/L的玉米浆;发酵条件为:温度30℃、接种量7%(v/v)、初始pH5.5、摇床转速120 r/min、装液量100 mL/250 mL.在此优化发酵培养基和发酵条件下,U-30发酵液中的乙醇浓度为39.04 g/L,乙醇得率为0.40 g/g,乙醇生产速率为0.325 g/L·h.以摇瓶中所得到的发酵条件为基础,在5 L发酵罐进行初步放大实验,发酵时间明显缩短为72 h,乙醇浓度达到39.875 g/L,乙醇生产速率为0.553 g/L·h.
Spathaspora passalidarum, a recently isolated native xylose-fermenting yeast strain, can ferment xylose faster than glucose, and can co-ferment glucose, xylose, and cellobiose. In this study, the fermentation conditions of a Spathaspora passalidarum mutant U-30 were investi-gated, including xylose content, the type and the content of nitrogen source, inoculation volume, shaking rate, and initial pH. The optimum fer-mentation conditions were summed up as follows:xylose of 100 g/L, corn steep of 15 g/L, temperature at 30℃, inoculation size of 7%(v/v), initial pH of 5.5, shaking rate of 120 r/min, and liquid volume 100 mL/250 mL. Under the above conditions, 39.04 g/L of ethanol was obtained, ethanol yield was 0.40 g/g, xylose utilization rate was about 100%, and ethanol productivity was 0.325 g/L·h. Further, the optimum fermenta-tion conditions in the shake flask was enlarged to 5 L fermentor. And the results showed that the fermentation time significantly shortened to 72 h, ethanol concentration reached up to 39.875 g/L, and ethanol productivity was as high as 0.553 g/L·h.
Spathaspora passalidarum, a recently isolated native xylose-fermenting yeast strain, can ferment xylose faster than glucose, and can co-ferment glucose, xylose, and cellobiose. In this study, the fermentation conditions of a Spathaspora passalidarum mutant U-30 were investi-gated, including xylose content, the type and the content of nitrogen source, inoculation volume, shaking rate, and initial pH. The optimum fer-mentation conditions were summed up as follows:xylose of 100 g/L, corn steep of 15 g/L, temperature at 30℃, inoculation size of 7%(v/v), initial pH of 5.5, shaking rate of 120 r/min, and liquid volume 100 mL/250 mL. Under the above conditions, 39.04 g/L of ethanol was obtained, ethanol yield was 0.40 g/g, xylose utilization rate was about 100%, and ethanol productivity was 0.325 g/L·h. Further, the optimum fermenta-tion conditions in the shake flask was enlarged to 5 L fermentor. And the results showed that the fermentation time significantly shortened to 72 h, ethanol concentration reached up to 39.875 g/L, and ethanol productivity was as high as 0.553 g/L·h.