학술논문
病毒性脑炎患者脑脊液和血清配对标本疱疹病毒和肠道病毒的检出率比较分析 / Comparison of detection rates of herpes virus and enterovirus in paired cerebrospinal fluid and serum specimens of patients with viral encephalitis
Document Type
Academic Journal
Author
申辛欣; 白雪丁; 黎赛; 阮洋; 易苏武; 肖政辉; 马学军; Shen Xinxin; Bai Xueding; Li Sai; Ruan Yang; Yi Suwu; Xiao Zhenghui; Ma Xuejun
Source
中华实验和临床病毒学杂志 / Chinese Journal of Experimental and Clinical Virology. 33(2):121-124
Subject
Language
Chinese
ISSN
1003-9279
Abstract
目的 比较病毒性脑炎患者脑脊液、血清配对标本中疱疹病毒属病毒,肠道病毒的检出率.方法 采集2017年12月至2018年2月湖南省长沙市儿童医院诊断疑似病毒性脑膜炎患儿109例脑脊液、血清配对标本,采用一步法巢式实时荧光定量RT-PCR和实时荧光定量PCR法分别进行肠道病毒和疱疹病毒属病毒检测,分析不同标本类型病毒检出率.运用SPSS17.0对检测结果进行统计分析.结果 109例患者的配对标本人疱疹病毒6型(human herpes virus type 6,HHV6)、单纯疱疹病毒(Herpes simplex virus,HSV)、EB病毒(Epstein-Barr virus,EBV)、巨细胞病毒(Cytomegalovirus,CMV)、肠道病毒(Enterovirus group A type 71,EV-A71)血清阳性率分别为7.34％、4.59％、7.34％、9.17％、10.09％,脑脊液阳性率分别为5.50％、2.75％、0、5.50％、6.42％.结果显示两种类型标本病毒检测结果的差异有统计学意义(P＜0.05).在脑脊液和血液样本中,可检出EV-A71病毒阳性的最长时间分别为发病后2d和7d,可检出CMV病毒阳性的最长时间分别为发病后3 d和26 d,可检出HHV6病毒阳性的最长时间分别为发病后7d和8d,可检出HSV1病毒阳性的最长时间均为发病后12 d;在血清样本中,可检出EBV病毒阳性最长时间为发病后10d,而脑脊液样本在发病10d内均无法检出该病毒.结论 EV-A71是导致该地区病毒性脑炎的最主要的病原体,血清标本病毒总体阳性率高于脑脊液标本.血清内脑炎相关的大多数病毒的存活时间较长于脑脊液中.提示对于病毒性脑炎患儿病原检测,时间具有重要意义,可根据发病时间合理选择标本类型进行测定.
Objective To compare the detection rate of herpes virus and enterovirus (EV) in paired cerebrospinal fluid and serum samples of patients with viral encephalitis.Methods A total of 109 paired cerebrospinal fluid and serum specimens were collected from patients who were clinically diagnosed with suspected viral meningitis in Children's Hospital of Hunan from December 2017 to February 2018.One-step nested real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR)and real-time fluorescence quantitative PCR were used to detect enterovirus and herpes virus respectively and the detection rates of different virus and sample types were analyzed.SPSS 17.0 was used for statistical analysis of the test result.Results Among the 109 pairs of specimens,the positive rates of human herpes virus type 6 (HHV6),herpes simplex virus-1 (HSV1),Epstein-Barr virus (EBV),cytomegalovirus (CMV) and enterovirus group A type 71(EV-A71) in serum were 7.34％,4.59％,7.34％,9.17％ and 10.09％,respectively,and in cerebrospinal fluid were 5.50％,2.75％,0,5.50％,and 6.42％,respectively.The result showed that there were statistically significant differences between the two types of specimens for herpes virus and enterovirus (P＜0.05).In cerebrospinal fluid and serum samples,the longest time for EV-A71 positive detection was 2 and 7 days after onset,respectively;the longest time for CMV positive detection was 3 and 26 days after onset,respectively;the longest time for HHV6 positive detection was 7 and 8 days after onset,respectively;the longest time for HSV1 positive detection was both 12 days after the onset;in serum samples,the longest time for EBV positive detection was10 days after onset,but in cerebrospinal fluid,no EBV was detected within 10 days of onset.Conclusions EV-A71 is the most prevalent pathogen causing viral encephalitis in hunan,the overall positive rate of virus in serum samples was higher than that in cerebrospinal fluid samples.Virus stays longer in serum than in cerebrospinal fluid.It is suggested that the time is of great significance for the pathogen detection of children with viral encephalitis,the specimen type can be selected reasonably according to the time of onset.
Objective To compare the detection rate of herpes virus and enterovirus (EV) in paired cerebrospinal fluid and serum samples of patients with viral encephalitis.Methods A total of 109 paired cerebrospinal fluid and serum specimens were collected from patients who were clinically diagnosed with suspected viral meningitis in Children's Hospital of Hunan from December 2017 to February 2018.One-step nested real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR)and real-time fluorescence quantitative PCR were used to detect enterovirus and herpes virus respectively and the detection rates of different virus and sample types were analyzed.SPSS 17.0 was used for statistical analysis of the test result.Results Among the 109 pairs of specimens,the positive rates of human herpes virus type 6 (HHV6),herpes simplex virus-1 (HSV1),Epstein-Barr virus (EBV),cytomegalovirus (CMV) and enterovirus group A type 71(EV-A71) in serum were 7.34％,4.59％,7.34％,9.17％ and 10.09％,respectively,and in cerebrospinal fluid were 5.50％,2.75％,0,5.50％,and 6.42％,respectively.The result showed that there were statistically significant differences between the two types of specimens for herpes virus and enterovirus (P＜0.05).In cerebrospinal fluid and serum samples,the longest time for EV-A71 positive detection was 2 and 7 days after onset,respectively;the longest time for CMV positive detection was 3 and 26 days after onset,respectively;the longest time for HHV6 positive detection was 7 and 8 days after onset,respectively;the longest time for HSV1 positive detection was both 12 days after the onset;in serum samples,the longest time for EBV positive detection was10 days after onset,but in cerebrospinal fluid,no EBV was detected within 10 days of onset.Conclusions EV-A71 is the most prevalent pathogen causing viral encephalitis in hunan,the overall positive rate of virus in serum samples was higher than that in cerebrospinal fluid samples.Virus stays longer in serum than in cerebrospinal fluid.It is suggested that the time is of great significance for the pathogen detection of children with viral encephalitis,the specimen type can be selected reasonably according to the time of onset.