학술논문
浙江省不同宿主来源狂犬病病毒N基因分子特征分析 / Sequencing and analysis of N gene of street rabies virus isolated from different hosts in Zhejiang province
Document Type
Academic Journal
Source
中华流行病学杂志 / Chinese Journal of Epidemiology. 35(7):837-841
Subject
Language
Chinese
ISSN
0254-6450
Abstract
目的 测定浙江省不同宿主(人、鼬獾、犬)来源的狂犬病病毒街毒株N基因序列,分析病毒遗传变异特征及其与流行的关系.方法 采用直接免疫荧光试验和反转录聚合酶链式反应检测狂犬病病毒阳性标本N基因核苷酸序列,利用生物信息学软件分析基因序列和编码蛋白.结果 共获得浙江省2个人源、5个鼬獾源和11个犬源狂犬病病毒街毒株N基因核苷酸序列,18个核苷酸和氨基酸序列同源性在89.7％～ 100.0％和98.4％～ 100.0％,N蛋白一级结构上绝大部分为稳定区域,编码基因的核苷酸变异多为无义突变,系统发育分析显示18个街毒株均属于传统的基因1型.结论 浙江省不同宿主来源狂犬病病毒的流行具有地域性特征,同类宿主动物病毒株或来自同一县域/相邻县域的毒株在地理位置上最为近缘,但人源株病毒更具有复杂性.浙江省狂犬病病毒街毒株的流行具有通过犬向鼬獾和人传播,并在犬、鼬獾中跨区域循环传播的特点.
Objective To elucidate the characteristics of genetic variability and its relationship with prevalence,through sequencing and analysis of N gene among street rabies virus isolated from different hosts (homo sapiens,ferret badger,dog) in Zhejiang province.Methods Samples were screened and confirmed by direct fluorescence assay and reverse transcript PCR.Sequences were analyzed using bio-information software.Results Eighteen street rabies virus strains were identified,including 2 from homo sapiens,5 from ferret badger,and 11 from dog.Similarities of N gene and N protein were calculated to be 89.7％-100.0％ and 98.4％-100.0％ respectively.Mutations occurred in N gene were almost non-sense mutations.In addition,Data from phylogenetic analysis showed that all these strains could be classified into traditional genotype 1.Conclusion The prevalence of rabies viruses among different hosts in Zhejiang province had certain regional properties.Rabies viruses isolated from the same kind of host or from the same/adjacent county/ counties had the closest relationship.However,the characteristics of rabies virus prevalent in homo sapiens were somewhat complicated.In summary,the transmission of street rabies virus in Zhejiang province was from dogs to ferret badgers and homo sapiens,and the virus could circulate and cross-regional transmit among dogs and ferret badgers.
Objective To elucidate the characteristics of genetic variability and its relationship with prevalence,through sequencing and analysis of N gene among street rabies virus isolated from different hosts (homo sapiens,ferret badger,dog) in Zhejiang province.Methods Samples were screened and confirmed by direct fluorescence assay and reverse transcript PCR.Sequences were analyzed using bio-information software.Results Eighteen street rabies virus strains were identified,including 2 from homo sapiens,5 from ferret badger,and 11 from dog.Similarities of N gene and N protein were calculated to be 89.7％-100.0％ and 98.4％-100.0％ respectively.Mutations occurred in N gene were almost non-sense mutations.In addition,Data from phylogenetic analysis showed that all these strains could be classified into traditional genotype 1.Conclusion The prevalence of rabies viruses among different hosts in Zhejiang province had certain regional properties.Rabies viruses isolated from the same kind of host or from the same/adjacent county/ counties had the closest relationship.However,the characteristics of rabies virus prevalent in homo sapiens were somewhat complicated.In summary,the transmission of street rabies virus in Zhejiang province was from dogs to ferret badgers and homo sapiens,and the virus could circulate and cross-regional transmit among dogs and ferret badgers.