학술논문
党参茎叶液体发酵条件优化及成分变化分析 / Optimization of liquid fermentation condition and component change analysis of stems and leaves of Codonopsis
Document Type
Academic Journal
Author
张月荣; 姜霞; 赵俊禧; 杜晨晖; 王璞; 李伟; 詹海仙; ZHANG Yuerong; JIANG Xia; ZHAO Junxi; DU Chenhui; WANG Pu; LI Wei; ZHAN Haixian
Source
中国酿造 / China Brewing. 43(2):232-237
Subject
Language
Chinese
ISSN
0254-5071
Abstract
该试验以灵芝菌(Ganoderma lucidum)为菌种发酵党参茎叶,以多糖含量为响应值,通过单因素及响应面试验对党参茎叶液体发酵条件进行优化,并对发酵前后的党参茎叶成分变化进行分析.结果表明,党参茎叶液体发酵的最佳条件为发酵时间6.5 d、料液比7.5∶100(g∶mL)、培养基初始pH值8,在此条件下测得发酵党参茎叶粗多糖含量为37.59 mg/g.与发酵前相比,发酵党参茎叶中粗多糖含量增加115.32％,总皂苷含量降低56.22％,总黄酮含量降低77.33％.
In this experiment,using Ganoderma lucidum as strain,the stems and leaves of Codonopsis were fermented,using polysaccharide content as response value,the liquid fermentation conditions of stems and leaves of Codonopsis were optimized by single factor and response surface experi-ments,and the changes of stems and leaves of Codonopsis composition before and after fermentation were analyzed.The results showed that the optimal liquid fermentation conditions for stems and leaves of Codonopsis were fermentation time 6.5 d,solid to liquid ratio 7.5∶100(g∶ml),and the medium initial pH 8.Under these conditions,the content of crude polysaccharide in the fermented stems and leaves of Codonopsis was 37.59 mg/g.Compared with before fermentation,the crude polysaccharide content in the fermented stems and leaves of Codonopsis increased by 115.32％,the total saponins content decreased by 56.22％,and the total flavonoids content decreased by 77.33％.
In this experiment,using Ganoderma lucidum as strain,the stems and leaves of Codonopsis were fermented,using polysaccharide content as response value,the liquid fermentation conditions of stems and leaves of Codonopsis were optimized by single factor and response surface experi-ments,and the changes of stems and leaves of Codonopsis composition before and after fermentation were analyzed.The results showed that the optimal liquid fermentation conditions for stems and leaves of Codonopsis were fermentation time 6.5 d,solid to liquid ratio 7.5∶100(g∶ml),and the medium initial pH 8.Under these conditions,the content of crude polysaccharide in the fermented stems and leaves of Codonopsis was 37.59 mg/g.Compared with before fermentation,the crude polysaccharide content in the fermented stems and leaves of Codonopsis increased by 115.32％,the total saponins content decreased by 56.22％,and the total flavonoids content decreased by 77.33％.