학술논문
水辣蓼总黄酮的提取工艺及不同极性部位体外抑菌活性研究 / Study on Extraction Technology of Total Flavonoids from Polygonum hydropiper and Antimicrobial Susceptibility of Different Polarity Fractions
Document Type
Academic Journal
Author
Source
中国现代中药 / Modern Chinese Medicine. 19(6):839-844
Subject
Language
Chinese
ISSN
1673-4890
Abstract
目的:优化水辣蓼总黄酮的超声提取工艺,比较不同极性萃取物对致病性大肠杆菌的体外抑菌效果.方法:在单因素试验的基础上,选取料液比、超声时间及乙醇浓度三因素进行Box-Behnken响应面设计.采用牛津杯法测定各萃取物的抑菌圈直径,通过微量稀释法和平板法测定最低抑菌浓度(MIC).结果:水辣蓼总黄酮最优提取条件为料液比1∶38,乙醇浓度66%,超声提取3次,每次37 min;总黄酮质量分数为6.084 7 mg·g-1.不同极性的萃取物中,乙酸乙酯部位抑菌活性最强,抑菌圈直径(136.8±0.08)mm,最低抑菌浓度(MIC)为25 mg·mL-1.结论:优化的提取工艺稳定可行、操作简便;水辣蓼的乙酸乙酯萃取物是其体外抑制致病性大肠杆菌的活性部位.
Objective:To optimize the extraction conditions of total flavonoids from Polygonum.hydropiperL.and compare the antimicrobial susceptibility of different extracts on pathogenic E.coli. Methods:On the basis of single-factor tests,Box-Behnken Design was used to optimize extraction technology of total flavonoids from P.hydropiperwith three factors of liquid-solid ratio,ethanol concentration and extraction time.The diameter of inhibition zone of different extracts was determined by cylinder plate method and minimal inhibitory concentration (MIC) was investigated by the broth dilution and plate method. Results:The optimal extraction conditions were as follows:ultrasonic extracting with 66% ethanol for three times,37 min for each time,liquid-solid ratio of 38∶1(mL∶g).Under the optimal condition,the content of total flavonoids was 6.084 7 mg·g-1.Among the extracts with different polarities,ethyl acetate extract showed the strongest antibacterial activity,with the inhibition zone diameter of 136.8±0.08 mm,and MIC of 25 mg·mL-1. Conclusion:The optimum extraction process is stable,simple and feasible.The ethyl acetate extract could be considered as the antibacterial active site of P.hydropiper.
Objective:To optimize the extraction conditions of total flavonoids from Polygonum.hydropiperL.and compare the antimicrobial susceptibility of different extracts on pathogenic E.coli. Methods:On the basis of single-factor tests,Box-Behnken Design was used to optimize extraction technology of total flavonoids from P.hydropiperwith three factors of liquid-solid ratio,ethanol concentration and extraction time.The diameter of inhibition zone of different extracts was determined by cylinder plate method and minimal inhibitory concentration (MIC) was investigated by the broth dilution and plate method. Results:The optimal extraction conditions were as follows:ultrasonic extracting with 66% ethanol for three times,37 min for each time,liquid-solid ratio of 38∶1(mL∶g).Under the optimal condition,the content of total flavonoids was 6.084 7 mg·g-1.Among the extracts with different polarities,ethyl acetate extract showed the strongest antibacterial activity,with the inhibition zone diameter of 136.8±0.08 mm,and MIC of 25 mg·mL-1. Conclusion:The optimum extraction process is stable,simple and feasible.The ethyl acetate extract could be considered as the antibacterial active site of P.hydropiper.