학술논문
落叶松林建群种差异对土壤真菌多样性的影响 / Effects of constructive species difference on soil fungal diversity in Larix gmelini forests
Document Type
Academic Journal
Author
Source
中南林业科技大学学报 / Journal of Central South University of Forestry & Technology. 43(12):153-164
Subject
Language
Chinese
ISSN
1673-923X
Abstract
[目的]探究寒温带落叶松林建群种差异对土壤真菌多样性的影响,以期为落叶松林群落构建和土壤真菌多样性的维持机制研究提供依据.[方法]在大兴安岭落叶松林监测样地内进行植被调查,计算落叶松的重要值并以此为实验处理,采用Illumina MiSeq高通量测序技术对土壤进行测序,解析建群种差异下土壤真菌的群落组成和多样性的变化特征,并探讨影响其变化的土壤环境因素.[结果]落叶松林的土壤理化性质具有显著差异(P＜0.05),其中土壤AP含量随落叶松重要值升高而显著升高,SOC、MBC、pH值和C/N为显著下降.测序获得土壤真菌14门47纲96目176科298属463种,其中担子菌门和子囊菌门为优势真菌门,除毛霉门外,其余菌门的相对丰度具有显著差异(P＜0.05).各落叶松林的优势属存在不同,并且各菌属的相对丰度差异显著(P＜0.05).Chao1、Shannon和Simpson指数结果表明落叶松林土壤真菌的Alpha多样性无显著差异(P＞0.05),NMDS分析表明落叶松林土壤真菌Beta多样性具有极显著差异(P=0.001),土壤pH值、AN、MBC、C/N、SOC、TN、AP和WC是土壤真菌群落结构差异显著的影响因子.功能预测结果表明,土壤真菌以共生型、腐生型和腐生-共生型为主要营养方式,外生菌根真菌为最主要的功能群,其相对丰度随叶松重要值的升高而显著降低(P＜0.05).[结论]建群种重要值差异的落叶松林土壤真菌群落组成、多样性和潜在功能具有显著差异,土壤理化性质的差异是其主要的影响因素.
[Objective]This study aims to explore the impacts of differences in constructive species on soil fungal diversity in cold temperate Larix gmelinii forests,which is expected to provide useful reference for the construction of L.gmelinii forest communities and the maintenance of soil fungal diversity.[Method]The vegetation survey was conducted in the monitoring plot of larch forests in the Greater Khingan Mountains,the important values of L.gmelinii were calculated and used as the experimental treatments.The soil was sequenced by Illumina MiSeq high-throughput sequencing technology to analyze the changes of soil fungal community composition and diversity under the difference of constructive species,and to explore the soil environmental factors affecting its changes.[Result]The soil physical and chemical properties of L.gmelini forests were significantly difference(P＜0.05).The soil AP contents increased significantly with the increase of L.gmelini important value,while soil SOC,MBC,pH and C/N showed a significant downward trend.A total of 463 species of soil fungi belonging to 14 phyla,47 classes,96 orders,176 families and 298 genera were obtained by sequencing.Basidiomycota and Ascomycota were the dominant fungal phyla.Except for Mucoromycota,the relative abundance of other phyla was significantly different(P＜0.05).The dominant genera of larch forests were different,and the relative abundance of each genus was significantly difference(P＜0.05).The results of Chao1,Shannon and Simpson indexes showed that there was no significant difference in the Alpha diversity of soil fungi in L.gmelini forest(P＞0.05).NMDS analysis showed that the Beta diversity of soil fungi was significantly difference(P=0.001).Soil pH,AN,MBC,C/N,SOC,TN,AP and WC were the influencing factors of significant differences in soil fungal community structure.The results of functional prediction showed that the main trophic modes of soil fungi were Symbiotroph,Saprotroph and Saprotroph-Symbiotroph,and Ectomycorrhizal fungi were the most important functional groups.As increase of the important value of L.gmelinii,the Ectomycorrhizal decreased significantly(P＜0.05).[Conclusion]The composition,diversity and potential function of soil fungal community in L.gmelinii forests with different important values of constructive species were significantly different,and the difference of soil physical and chemical properties was the main influencing factor.
[Objective]This study aims to explore the impacts of differences in constructive species on soil fungal diversity in cold temperate Larix gmelinii forests,which is expected to provide useful reference for the construction of L.gmelinii forest communities and the maintenance of soil fungal diversity.[Method]The vegetation survey was conducted in the monitoring plot of larch forests in the Greater Khingan Mountains,the important values of L.gmelinii were calculated and used as the experimental treatments.The soil was sequenced by Illumina MiSeq high-throughput sequencing technology to analyze the changes of soil fungal community composition and diversity under the difference of constructive species,and to explore the soil environmental factors affecting its changes.[Result]The soil physical and chemical properties of L.gmelini forests were significantly difference(P＜0.05).The soil AP contents increased significantly with the increase of L.gmelini important value,while soil SOC,MBC,pH and C/N showed a significant downward trend.A total of 463 species of soil fungi belonging to 14 phyla,47 classes,96 orders,176 families and 298 genera were obtained by sequencing.Basidiomycota and Ascomycota were the dominant fungal phyla.Except for Mucoromycota,the relative abundance of other phyla was significantly different(P＜0.05).The dominant genera of larch forests were different,and the relative abundance of each genus was significantly difference(P＜0.05).The results of Chao1,Shannon and Simpson indexes showed that there was no significant difference in the Alpha diversity of soil fungi in L.gmelini forest(P＞0.05).NMDS analysis showed that the Beta diversity of soil fungi was significantly difference(P=0.001).Soil pH,AN,MBC,C/N,SOC,TN,AP and WC were the influencing factors of significant differences in soil fungal community structure.The results of functional prediction showed that the main trophic modes of soil fungi were Symbiotroph,Saprotroph and Saprotroph-Symbiotroph,and Ectomycorrhizal fungi were the most important functional groups.As increase of the important value of L.gmelinii,the Ectomycorrhizal decreased significantly(P＜0.05).[Conclusion]The composition,diversity and potential function of soil fungal community in L.gmelinii forests with different important values of constructive species were significantly different,and the difference of soil physical and chemical properties was the main influencing factor.