학술논문
腺苷酸活化蛋白激酶对缺氧肾小管上皮细胞线粒体片段化的影响 / The Role of AMP-activated Protein Kinase On Mitochondrial Fragmentation of Renal Tubular Epithelial Cells Under Hypoxia
Document Type
Academic Journal
Author
曾红惠; 汤颖; 陈俊哲; 余文娟; 黄秋燕; 许燕纯; 徐安平; ZENG Hong-hui; TANG Ying; CHEN Jun-zhe; YU Wen-juan; HUANG Qiu-yan; XU Yan-chun; XU An-ping
Source
岭南急诊医学杂志 / Lingnan Journal of Emergency Medicine. 24(2):110-113
Subject
Language
Chinese
ISSN
1671-301X
Abstract
目的:探讨腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)缺氧环境中对肾小管上皮细胞线粒体片段化的影响.方法:采用AnaeroPack System建立缺氧模型,在缺氧前加入Compound C抑制AMPK活性.缺氧培养肾小管上皮细胞48小时后,蛋白免疫印迹法检测AMPK磷酸化水平,并用线粒体示踪染料标记线粒体后在共聚焦显微镜下观察线粒体形态,并统计线粒体片段化细胞的数目及比例.结果:缺氧后肾小管上皮细胞线粒体片段化明显,伴有AMPK磷酸化水平显著升高.予以Compound C抑制AMPK活性后,肾小管上皮细胞线粒体片段化显著减少.结论:缺氧肾小管上皮细胞的线粒体片段化与AMPK密切相关,减少AMPK磷酸化可减轻缺氧肾小管上皮细胞线粒体片段化,保护细胞.