학술논문
禽流感病毒感染小鼠前后表面蛋白编码基因变异情况分析 / Variations in surface protein genes of avian influenza virus before and after infecting mouse
Document Type
Academic Journal
Author
冼盈; 温景芸; 张扣兴; 廖明; 罗开健; 任涛; 辛朝安; XIAN Ying; WEN Jing-yun; ZHANG Kou-xing; LIAO Ming; LUO Kai-jian; REN Tao; XIN Chao-an
Source
中华临床感染病杂志 / CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES. 2(2):93-97
Subject
Language
Chinese
ISSN
1674-2397
Abstract
目的 了解禽流感病毒(AIV)在感染小鼠前后表面蛋白编码基因的特点及变异情况.方法 在A/Goose/Guangdong/NH/2003(H5N1)感染小鼠肺组织的第12小时和第9天各分离1株AIV病毒,通过鸡胚增殖后提取RNA,反转录合成cDNA,经PCR扩增和产物纯化构建重组质粒,用双脱氧链终止法进行核苷酸序列测定并进行基因特性分析.结果 3株病毒HA基因的核苷酸序列同源性为99.6%～99.8%,推导氨基酸序列的同源性为99.3%～99.6%.3株病毒NA基因的核苷酸序列同源性为99.8%～99.9%,均为同义突变.M基因未发生任何变异.结论 AIV感染小鼠后HA基因出现变异,NA和M基因未出现有意义的突变.
Objective To investigate the variations of surface protein genes of avian influenza virus (AIV)before and after infecting mouse.Methods Mouse lung tissue was infected with A/Goose/Guangaong/NH/2003(H5N1)and the virus was isolated 12 hours and 9 days after replication in lung tissue of mouse.The isolated strains were amplified in embryonated chicken eggs,anti the virion RNA was transcribed into cDNA by reverse transeriptase.After amplification and purification,dideoxy-mediated chain termination was performed to detect synthetic oligonucleotide primers and DNA sequence was analyzed.Results The homology of nucleotide sequence for HA gene of three isolated strains was 99.6%-99.8%.and that of amino acid sequences was 99.3%-99.6%.The homology of nucleotide sequence for NA gene of three strains was 99.8%-99.9%.all of them were synonymous mutatinns.No variation was found in M gene.Conclusion After replication in mouse lung tissue,no significant mutation was found in the surface protein genes of AIV except some point mutations in HA genes.
Objective To investigate the variations of surface protein genes of avian influenza virus (AIV)before and after infecting mouse.Methods Mouse lung tissue was infected with A/Goose/Guangaong/NH/2003(H5N1)and the virus was isolated 12 hours and 9 days after replication in lung tissue of mouse.The isolated strains were amplified in embryonated chicken eggs,anti the virion RNA was transcribed into cDNA by reverse transeriptase.After amplification and purification,dideoxy-mediated chain termination was performed to detect synthetic oligonucleotide primers and DNA sequence was analyzed.Results The homology of nucleotide sequence for HA gene of three isolated strains was 99.6%-99.8%.and that of amino acid sequences was 99.3%-99.6%.The homology of nucleotide sequence for NA gene of three strains was 99.8%-99.9%.all of them were synonymous mutatinns.No variation was found in M gene.Conclusion After replication in mouse lung tissue,no significant mutation was found in the surface protein genes of AIV except some point mutations in HA genes.