학술논문
STR Data for the PowerPlex® 16 System Loci for the Malays, Chinese and Indians Groups of the Malaysian Population
Document Type
journal paper
Author
Source
Journal of Forensic Sciences, Sep 2005, Vol. 50, No. 5, pp. JFS2005170-4.
Subject
Language
English
ISSN
0022-1198
Abstract
A STR database for use in forensic casework was created for three major ethnic population groups in Malaysia. Blood samples were collected from unrelated individuals and DNA were extracted using Chelex method (1). PCR amplification was performed following manufactures's instructions (PowerPlex® 16 System, Promega Corporation). The amplified products were analyzed and detected using the ABIPRISM® 3100 Gene Analyzer (Applied Biosystem) with 3100 Data Collection Software (Version 1.1). The results were analyzed with GeneScan analysis software (Version 3.7.1) and genotypes were determined by comparison to allelic ladder (Promega Corporation) using Genotyper DNA fragment analysis software (Version 3.7) with the PowerTyperTM Macros (Promega Corporation).