부산대학교 도서관

In this study, Colocasia esculenta (L.) Schott was used as tissue culture material to investigate the effect of plant growth regulators on shoot proliferation and rooting, and the γ-radiation treatment. The results showed that adding 6-benzylaminopurine (BA) or Kinetin to the medium could effectively promote the proliferation of buds, and the proliferation number of buds with 0.5, 1.0 mg/L BA and 1.0 mg/L Kinetin were 2.5, 2.6 and 2.1, respectively, which were significantly higher than that of without cytokinin. In addition, adding different concentrations of BA (0.5, 1.0, 1.5, 2.0, 3.0 and 5.0 mg/L) could promote the proliferation of buds, and the proliferation number of buds was between 2.3 and 2.9. However, there was no significant difference among these concentrations. In terms of shoot rooting, number of 12.5 with 1.0 mg/L IBA (indole butyric acid) and 14.5 with 0.1 mg/L NAA (1-Naphthaleneacetic acid) were obtained, which were significantly higher than rooting number of 7.8 from the auxin-free control. After irradiating in vitro proliferated buds with γ-rays (0, 5, 10, 15, 20, and 25 Gy), the survival rate and variation rate were recorded. It was found that dosage of 15 Gy provides best survival rate of 99.6% and the variation rate of 27.7%. Nevertheless, the survival rate of 20 Gy already reached the half-lethal dose, showing that the suitable irradiation dose of γ-ray may be in the range of 15-20 Gy.