부산대학교 도서관

The study aims to detect biofluid-specific metabolites by LC-Q-ToF mass spectrometer for forensic biofluid identification. Firstly, the applicability of 6 solvents for extraction of 6 different biofluids (urine, semen, venous blood, menstrual blood, saliva and sweat), each of them sourced from 6 individuals, were evaluated. The most abundant metabolites except for the menstrual blood were observed by using methanol/acetone (v/v 3:7) as extraction solvent, and it was thus taken as the extraction solvent in the following experiments. Among these 6 different biofluids, the most abundant metabolites were observed in semen (81.83 ± 9.45) while in saliva was the least (42.50 ± 3.39). Furthermore, metabolomic mutuality ratio for inter-individual similarity showed the lowest percentage for sweat (29.96 ± 4.65%) and the highest for saliva (52.03 ± 3.98%). For biofluid-specific metabolite markers searching, 3 urine-specific metabolites, 6 semen-specific metabolites and 1 blood-specific metabolic markers were observed. Additionally, 1 metabolite could be used to distinguish between venous and menstrual blood. All metabolites claimed above to be biofluid-specific markers showed reproducibility in both intra-individual and inter-individual tests, and no inter-biofluid false-positive results were observed. The human biofluid identification system established in this study by LC-Q-ToF mass spectrometry could be a potential technique for practical forensic biofluid identification.