부산대학교 도서관

During Dec. 2019 to Feb. 2020, the leaves of strawberry with red brown to dark brown lesions, the symptom looked like anthracnose. The fruits of strawberry with purple, brown, white and water-soaked symptoms, at later stage, it will appear white mycelium. The infected leaves and fruits of strawberry were collected from Dahu strawberry farm, Miaoli county, and the green house in CH Biotech R ＆ D Co., Nantou city, LTD. At the same time, the fruits of wax apple with purple and water-soaked symptoms were collected from Linbian township, Pingtung county. The pathogens of leaves and fruits of strawberry and wax apple were isolated, purified and colonized to observe the spores, and inoculation. The pathogens of strawberry (SL) and wax apple (WF) were identified as Neopestalotiopsis rosae and Neopestalotiopsis sp., respectively, based on the combined sequences of internal transcribed spacer, β-tubulin and transcription elongation factor. Neopestalotiopsis were reported infecting the strawberry leaf, fruit, crown and root in China, Spanish and Brazil, however N. rosae was identified in Taiwan for the first time. In this study, N. rosae and the pathogen of wax apple fruit rot were inoculated on detached leaves of three different strawberry varieties. The result showed 'Aroma' was susceptible, 'Tokun' was moderate resistant, and 'Taoyuan No.1' was resistant. N. rosae. could infect the leaf of 'Aroma' by inoculated the concentration of 10^4 conidia/ml. The pathogen of wax apple fruit rot could not infect three different strawberry varieties with the concentration of 10^4 and 10^5 conidia/ml. If the inoculated concentration increased to 10^6conidia/ml, the pathogen of wax apple could infect three different strawberry varieties. It showed that pathogen of wax apple could not infect the leaf of strawberry easily, and 'Aroma' is the most susceptible variety. The farmers change the cultivated varieties into 'aroma'. It may cause N. rosae. damaged more intense. Moreover, growth inhibition rate of fungicides were tested. The result showed that 25% prochloraz EC, 25.9% folicur EW, 75 g/L epoxiconazole EC, 39.5% fluazinam SC, 80% mancozeb WP, and 62.5% cyprodinil + fludioxonil WG had better suppression effect, and the growth inhibition rate could reach up to 90%. Only 62.5% cyprodinil + fludioxonil WG was recommended to control the strawberry Botrytis disease in Plant Protection Information System.