부산대학교 도서관

Chrysanthemum (Chrysanthemum morifollium Ramat.) is a perennial crop belonging to the Asteraceae family, and one of the primary cut-flower crops in Taiwan. This study surveyed the plant parasitic nematodes (PPNs) in the major chrysanthemum planting regions in Taiwan. Chunghua and Chiayi counties are primary cut-flower chrysanthemum cultivated region, but Miaoli and Taitung counties grow 'Hang' chrysanthemum for health supplements. Eight genus of plant parasitic nematodes identified in this study were Rotylenchulus, Pratylenchus, Paratylenchus, Trichodorus, Helicotylenchus, Tylenchorhynchus, Hirschmanniella, and Meloidogyne. In Miaoli and Chiayi counties, the reniform nematode was the dominant PPN population. In Chunghua and Chiayi counties, the root lesion nematode and pin nematode were the predominate populations. Soil samples at fields TW-1, TW-2, TW-3 after they rotated with rice, and TW-4, TW-8 after they practiced fallow showed that the PPNs populations in all fields decreased to an undetectable level. Four field populations were selected for mass cultivation, and processed for identification. Morphology and morphometric data as well as rDNA ITS sequences of the populations were obtained. Two populations in Miaoli and Chiayi (ML-2, CY-2) were identified as Rotylenchulus reniformis. The populations in Chunghua were identified as Pratylenchus kumamotoensis (TW-2), Paratylenchus dianthus (TW-4), respectively, and were two new recorded species in Taiwan. When challenged six hundred P. kumamotoensis on different chrysanthemum (2 all stage / ml soil treatment) cv. 'Hang' showed obvious lesion on roots, the treatment also had lowest shoot weight. This study indicates this plant parasitic nematode has high potential to cause severe damage on monocropping chrysanthemum cultivation regions.