부산대학교 도서관

In the last few decades, Bovine spongiform encephalopathy (BSE) and other food safety issues have aroused consumers' concern. EU member countries and other nations have regulations to ensure food traceability in order to promote food safety. Molecular traceability using DNA markers has become popular in recent years. Microsatellite markers have many advantages and are thus often utilized as molecular markers in molecular traceability. The objective of this study is to establish the microsatellite marker sets for genetic verification traceability of pure and hybrid pigs and fresh pork in Taiwan. In this study, purebred pigs of three breeds, total 212 heads (41 Landrace, 95 Yorkshire and 76 Duroc) from the central test station and 46 heads commercial crossbred pigs (27 Landrace×Duroc, LD, 19 Landrace×Yorkshire×Duroc, LYD), were genotyped using 10 microsatellite marker sets. We performed two multiplex polymerase chain reaction (PCR) with fluorescent-labeled microsatellite markers. The results showed that the expected heterozygosity (H E) in genetically unrelated individuals of three pure breeds were 0.62, 0.65 and 0.65, respectively; while that in all individuals (with partial genetic relationship) of three pure breeds were 0.61, 0.66 and 0.65, respectively. The polymorphism information content (PIC) in unrelated individuals of the three breeds were 0.57, 0.60 and 0.60, respectively; in all individuals (partial genetic relationship) of three breeds were 0.57, 0.61 and 0.61. After performing Hardy-Weinberg equilibrium (HWE) test in the samples, in unrelated individuals of the three breeds, there were one microsatellite marker in Landrace, one marker in Yorkshire and three markers in Duroc against HWE, respectively (P ＜ 0.05); in all individuals (partial genetic relationship) of three breeds, there were one marker in Landrace, two markers in Yorkshire and three in Duroc against HWE, respectively (P＜0.05). The results of individual identification showed that total probability of identify (tP (ID)) in unrelated individuals of three breeds were 1.42×10 -8 , 6.49×10 -9 and 1.23×10 -8 , respectively; in all individuals (partial genetic relationship) of three breeds were 1.58×10 -8 , 5.61×10 -9 and 9.34×10 -9 , respectively. The tP (ID) of LD and LYD crossbred pigs were 1.79×10 -9 and 6.14×10 -9, respectively. All the tested groups had their tP (ID) or probability of identify in sibs (tP (ID) sibs) values less than 1.00×10 -7. In Taiwan, the annual number of purebred and crossbred pigs slaughtered was less than 10 million (1×10 7) heads in the past five years. Thus, according to the results of this study, 10 microsatellite markers are sufficient for individual identification among the common purebred and crossbred commercial pigs in the molecular traceability of purebred and crossbred pigs as well as their fresh pork.