부산대학교 도서관

Banana (Musa sp.) is cultivated worldwide and is one of the most popular fruits. Based on the statistics of FAO (Food and Agriculture Organization of the United Nations, Rome, Italy), the banana was the second most important fruits in the world. Accurate methods for the identification and characterization of pathogens provide the basis of integrated pest management strategies to counteract diseases. Fusarium oxysporum f. sp. cubense (Foc), a soil-borne fungal pathogen, can result in Fusarium wilt of banana (FWB), which limits harvest amounts of banana. To prevent the outbreak of FWB, and also, dwindle the lost of economic loss of banana yield, it is pivotal to develop a rapid method with specific and fast for disease management. This study used the molecular detection methods (Foc race 4 PCR and Foc TR4 PCR) based on the polymerase chain reaction (PCR) assays for rapid detection of Foc race 4 (including tropical race 4 (TR4) and subtropical race 4 (ST4), which can currently affect bananas in the most banana-producing regions of the world. The detection limit of the Foc race 4 PCR assay (or Foc TR4 PCR assay) was estimated to be 10^5 (or 10^6) copy of the target standard DNA; 10 (or 100) pg of the Foc genomic DNA; 1μg mycelium and 100 spores of Foc. The Foc PCR assays were suitable for the molecular detection of Foc-infected banana pseudostems collected in the field. In addition, a total of 212 banana pseudostem samples infected with Foc from different geographic locations in Taiwan were performed for plate-out assay and the molecular detection assays. The detection rates of Foc race 4 and Foc TR4 were 212/212 (100%) and 209/212 (98.6%), respectively. The results indicated that the Foc TR4 was dominant in the Foc population in Taiwan. The Foc PCR assays have the potential to serve as a rapid and specific tool for the routine Foc detection.