부산대학교 도서관

Beauveria bassiana (BA) is a fungal pesticide for controlling many kinds of insects in the field or indoors. The purpose of this work is to evaluate the allergenicity of crude antigen of BA in Sprague-Dawley rats. Rats were intratracheally (IT) challenged with 12 μg crude antigen after 14 days treatment by intraperitoneal sensitization with the crude antigen (0, 3.25, 7.5, 15 μg) in adjuvant. Additionally, rats were sensitized with adjuvant alone as control group. The bronchoalveolar lavage (BAL) fluid were harvested at 1 and 7 days post IT challenge (DPIT), and then measured total cell counts, eosinophils, IgE, IL-4, total protein and LDH activity. The results showed that the total cell counts, eosinophils, IgE, and IL-4 significantly increased in medium and high doses of crude antigen-sensitized rats at both 1 and 7 DPIT as compared with adjuvant controls. However the total protein and LDH concentrations had no significant differences between treated and control animals. Besides, histopathology micrographs of rat lungs showed alveolar spaces, perivascuar and peribrochial infiltration in rats sensitized with crude antigen of Beauveria bassiana at high dose. These data demonstrated that the crude antigen of Beauveria bassiana might have the latent allergenicity to the SD rats.