학술논문
Isolation and Characterization of Microsatellite Loci from Pinus Massoniana (Pinaceae) / 馬尾松（松科）微衛星基因座的分離及分析
Document Type
Article
Author
洪國翔 / Kuo-Hsiang Hung; 林其永 / Chi-Yung Lin; 黃啟俊 / Chi-Chun Huang; 黃吉川 / Chi-Chuan Hwang; 許再文 / Tsai-Wen Hsu; 郭耀綸 / Yau-Lun Kuo; 王唯匡 / Wei-Kuang Wang; 洪承裕 / Cheng-Yu Hung; 蔣鎮宇 / Tzen-Yuh Chiang
Source
Botanical Studies / 植物學研究. Vol. 53 Issue 2, p191-196. 6 p.
Subject
Language
英文
ISSN
1817-406X
Abstract
Pinus massoniana is widespread in the central and eastern mainland China, while distributed in Taiwan with a single population. This island population has been declining due to habitat destruction and pine wilt disease. In the study we isolated microsatellite loci from P. massoniana for investigating the population structure. Eleven, novel microsatellite markers were developed from P. massoniana by using a modified PCR-based isolation of microsatellite arrays (PIMA) method. The number of alleles, observed and expected heterozygosities across loci varied with a range of 2-9, 0.00-0.82 and 0.40-0.83, respectively. The application of these microsatellite markers of P. massoniana provides a tool for understanding demography and population structure in Taiwan and mainland China.