부산대학교 도서관

Platelet aggregation test can be used to evaluate the platelet aggregation function for the diagnosis of inherited platelet function abnormal diseases. However, the reference intervals of platelet aggregation test were not yet well established in Taiwan due to difficult sample collection and complicated examination procedure. To establish the reference intervals of platelet aggregation for Taiwanese population, platelet-rich plasma (PRP) and platelet-poor plasma (PPP) specimens were collected from 125 healthy adults and analyzed by BIO/DATA Aggregation Profiler aggregometer. In this study, four kinds of agonists were used, including Ristocetin (0.3-1.5 mg/mL), ADP (2-20 μM), Collagen (1-5 μg/mL), Epinephrine (1-100 μM). Results were statistical analyzed by kolmogorov-smirnov test using the SPSS software and were shown as final aggregation rate (%). The reference intervals of each agonists with different concentration were established as Ristocetin 1.5 mg/mL (84.43-97.23%), Ristocetin 1.2 mg/mL (81.38-93.82%), Ristocetin 1.0 mg/mL (56.58-93.54%), Ristocetin 0.6 mg/mL (3.64-9.12%), Ristocetin 0.3 mg/mL (4.32-8.96%), ADP 20 μM (72.80-88.42%), ADP 8 μM (62.93-86.95%), ADP 6 μM (57.50-81.98%), ADP 2 μM (9.81-69.91%), Collagen 5 μg/mL (78.98-92.10%), Collagen 3.3 μg/mL (72.47- 90.53%), Collagen 2.0 μg/mL (59.46-86.74%), Collagen 1.0 μg/mL (39.91-87.29%) and Epinephrine 100 μM (54.83-93.31%), Epinephrine 10 μM (51.82-94.59%) and Epinephrine 1 μM (11.91-67.07%), respectively. In conclusion, these results can not only be used for more accurate diagnosis but also be a reference for other laboratories.