부산대학교 도서관

A simple and fast method was developed for simultaneous determination of Acid blue 62 and other 12 colorants in cosmetics by liquid chromatography/tandem mass spectrometry (LC-MS/MS). The sample was uniformly dispersed with a solution of methanol: dichloromethane (8: 2, v/v) containing 0.1% ammonia water and then extracted by ultrasonication for 30 mins. After centrifugation and filtration, the filtrate was separated by a ACQUITY BEH Shield RP 18 column using gradient elution with 10 mM ammonium formate and methanol: acetonitrile (1: 1, v/v) solutions. The analysis could be accomplished within 15 mins by employing electrospray ionization (ESI) with the multiple reaction monitoring (MRM) mode for detection. The average recoveries of 13 compounds ranged from 87.7-113.2%, and the coefficients of variation were all less than 9.4%, by spiking multiple concentrations levels of standards ranging from 0.2-50 μg/g in lipstick samples. The matrix effects for ion enhancement or inhibition were less than 19.54%. This method can be employed to monitor the post-marketing products and further safeguard the health and safety of consumers.