부산대학교 도서관

A simple and rapid method was developed for simultaneous determination of 29 aromatic amines in hair dyes by liquid chromatography/tandem mass spectrometry (LC-MS/MS). The sample was uniformly dispersed with methanol containing 0.1% ammonia water and then extracted by ultrsonication for 30 min. After centrifugation and filtration, the filtrate was separated on a ZORBAX Eclipse XDB-C18 (3.5 µm, 2.1 mm x 50 mm) column by gradient elution of 5 mM ammonium acetate containing 0.05% formic acid and acetonitrile. The analysis can be accomplished within 15 min. by employing electrospray ionization (ESI) with multiple reaction monitoring (MRM) mode for detection. The average recoveries of 29 compounds ranged from 88.4-116.3%, and the coefficients of variation were all lower than 9.7%, when spiking multiple concentration levels of standards ranged from 0.02-400 μg/g in hair dye samples. The matrix effects for ion enhancement or inhibition were lower than 9.49%. This method can be applied to the monitoring of post-marketing products and safeguard the health and safety of the consumers.