부산대학교 도서관

Anastatus japonicus is a common egg parasitoid of Tessaratoma papillosa in Taiwan. In order to establish a mass rearing program for the parasitoid, this study examined the effects of low temperature (12 °C) storage on the survival of A. japonicus in the eggs of its factitious host, Samia cynthia. The results showed that the most suitable stage for the cold storage treatment was during the first five days of immature development. The emergence rates of parasitized eggs stored for up to 5 months were not significantly different from the untreated control group (64.8%). However, there was a significant reduction in the emergence rate for the eggs stored for 6 months. In addition, there was no significant difference in the parasitism rates among female A. japonicus wasps that emerged from 1-5 months of cold storage. The minimum number of progenies was 59.8 from the 7 to 13-day-old wasps with more than 71% of the progeny being female. The results of this study can be applied to the mass rearing of A. japonicus. Using the cold storage technique, the production of the biocontrol agent can start before T. papillosa becomes active in the spring. It provides the tool to achieve timely field release, which is essential to assure the effectiveness of T. papillosa biological control.